Detailed information of TA system TA077675

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 1927457..1928254
• Replicon: chromosome
• Accession: NZ_CP021851
• Organism: Enterobacter cloacae strain A1137

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: CCL06_RS09375
• Protein ID: WP_025755884.1
• Coordinates: 1927733..1928254 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A0F1BC27
• Locus tag: CCL06_RS09370
• Protein ID: WP_025755885.1
• Coordinates: 1927457..1927726 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCL06_RS09340	1923085..1923528	-	444	WP_025755890.1	helix-turn-helix transcriptional regulator	-
CCL06_RS09345	1923681..1924922	+	1242	WP_025755889.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
CCL06_RS09350	1924955..1925182	-	228	WP_008499832.1	YccJ family protein	-
CCL06_RS09355	1925201..1925797	-	597	WP_025755888.1	NAD(P)H:quinone oxidoreductase	-
CCL06_RS09360	1926190..1926360	+	171	WP_013097201.1	general stress protein	-
CCL06_RS09365	1926478..1927395	+	918	WP_025755886.1	DMT family transporter	-
CCL06_RS09370	1927457..1927726	+	270	WP_025755885.1	DUF1778 domain-containing protein	Antitoxin
CCL06_RS09375	1927733..1928254	+	522	WP_025755884.1	GNAT family N-acetyltransferase	Toxin
CCL06_RS09380	1928416..1929738	-	1323	WP_088244156.1	pyrimidine utilization transport protein G	-
CCL06_RS09385	1929760..1930254	-	495	WP_025755883.1	pyrimidine utilization flavin reductase protein F	-
CCL06_RS09390	1930264..1930854	-	591	WP_088244157.1	malonic semialdehyde reductase	-
CCL06_RS09395	1930864..1931664	-	801	WP_025755882.1	pyrimidine utilization protein D	-
CCL06_RS09400	1931672..1932058	-	387	WP_025755881.1	pyrimidine utilization protein C	-
CCL06_RS09405	1932070..1932759	-	690	WP_025755880.1	pyrimidine utilization protein B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-88)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19821.58 Da
• Isoelectric Point: 7.1163

>T77675 WP_025755884.1 NZ_CP021851:1927733-1928254 [Enterobacter cloacae]
VENLTIEMFSEGEEYDFRNFDCGEPSLNSFLADHLVRQHNGRILRGYLLKDRDHSRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYINVPSVTLGRLAVHKDLQGREWGTTLVTHAMRVVYLASQAVGVHGLFVDAINEQAKRFYLKLGFIPLTGENSHSLF
YPTKSIERLLEEE

Nucleotide

• Length: 522 bp

>T77675 NZ_CP021851:1927733-1928254 [Enterobacter cloacae]
GTGGAAAATCTCACGATCGAAATGTTTTCAGAGGGAGAAGAGTATGATTTCAGGAATTTCGATTGTGGTGAACCCTCGCT
AAATTCCTTCCTGGCAGACCATCTGGTGCGTCAGCACAATGGGCGTATTTTACGCGGCTATCTGCTTAAAGATCGCGACC
ATTCTCGTGTCCTGGGCTATTACACGCTGTCCGGAAGTTGCTTTGAAAAAGCGATGCTTCCTTCTAAGACTCAGCAGCGT
CGGATCCCTTATATCAACGTACCGAGCGTGACGCTGGGGCGTCTGGCTGTCCATAAAGACCTGCAGGGACGCGAGTGGGG
AACAACACTCGTCACGCATGCTATGCGCGTGGTTTACCTGGCATCCCAGGCGGTAGGCGTACATGGACTCTTTGTCGATG
CGATTAACGAACAGGCTAAACGGTTTTATCTGAAGCTGGGCTTTATCCCTCTGACGGGTGAAAACAGTCATTCGCTTTTT
TATCCCACCAAATCTATCGAACGCCTGCTTGAAGAAGAGTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10291.76 Da
• Isoelectric Point: 7.0174

>AT77675 WP_025755885.1 NZ_CP021851:1927457-1927726 [Enterobacter cloacae]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNETSWNAVMNAIDNPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT77675 NZ_CP021851:1927457-1927726 [Enterobacter cloacae]
ATGCCAGCACTTAAAAAACAGCGTATCGATCTTCGCCTGACGGACGAAGACAAAACAATGATTGAAGAGGCGGCGGCGAT
GACGAACCAAACAATCACCCAGTTTATGGTGAACAGTGCGTCTGAACGTGCTGCAGAGGTCATCGAACAGCATCGGCGTC
TCGTCTTAAACGAAACATCATGGAATGCCGTGATGAACGCCATTGACAATCCGCCTGAGCCAAATGAACGCCTGAAGCGA
GCGGCAAAACGTCTTCGAAACATGGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0F1BC27