Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1234035..1234661 Replicon chromosome
Accession NZ_CP021851
Organism Enterobacter cloacae strain A1137

Toxin (Protein)


Gene name Hha Uniprot ID A0A1H8SI38
Locus tag CCL06_RS05825 Protein ID WP_008499287.1
Coordinates 1234035..1234253 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag CCL06_RS05830 Protein ID WP_008499288.1
Coordinates 1234287..1234661 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCL06_RS05795 1230046..1230306 + 261 Protein_1103 type B 50S ribosomal protein L31 -
CCL06_RS05800 1230309..1230449 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
CCL06_RS05805 1230446..1231156 - 711 WP_025756156.1 GNAT family N-acetyltransferase -
CCL06_RS05810 1231258..1232718 + 1461 WP_088244093.1 PLP-dependent aminotransferase family protein -
CCL06_RS05815 1232690..1233157 - 468 WP_025756154.1 membrane protein -
CCL06_RS05820 1233275..1233826 - 552 WP_025756153.1 maltose O-acetyltransferase -
CCL06_RS05825 1234035..1234253 - 219 WP_008499287.1 hemolysin expression modulator Hha Toxin
CCL06_RS05830 1234287..1234661 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
CCL06_RS05835 1235173..1238319 - 3147 WP_047957901.1 multidrug efflux RND transporter permease subunit AcrB -
CCL06_RS05840 1238342..1239535 - 1194 WP_045285243.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T77672 WP_008499287.1 NZ_CP021851:c1234253-1234035 [Enterobacter cloacae]
MSDKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T77672 NZ_CP021851:c1234253-1234035 [Enterobacter cloacae]
ATGTCTGATAAACCACTCACTAAGATCGATTATTTGATGCGCCTGCGACGTTGTCAGTCAATTGACACCCTCGAACGCGT
CATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCCGCTGACCATCGTCTTGCTGAGC
TGACCATGAATAAGCTGTATGACAAAATCCCAACGTCGGTATGGAAATTTGTCCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT77672 WP_008499288.1 NZ_CP021851:c1234661-1234287 [Enterobacter cloacae]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT77672 NZ_CP021851:c1234661-1234287 [Enterobacter cloacae]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAACCTTGATGAAAGCAACCATGGCTGGGTAAACGACCCAACGTCTGCCATCAATTTACAGTTGAATGAGCTGATAGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GATGACACCTTTATGTTGTTCAGCAGTTACGGCATTAACGCCCAGGATTTGCAAAAATGGCGTAAATCGGGAAACCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1H8SI38

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References