Detailed information of TA system TA077671

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 624509..625025
• Replicon: chromosome
• Accession: NZ_CP021851
• Organism: Enterobacter cloacae strain A1137

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CCL06_RS03090
• Protein ID: WP_025759089.1
• Coordinates: 624741..625025 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0F1BEH9
• Locus tag: CCL06_RS03085
• Protein ID: WP_025759087.1
• Coordinates: 624509..624751 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCL06_RS03070	620604..621344	+	741	WP_025759082.1	KDGP aldolase family protein	-
CCL06_RS03075	621365..622501	+	1137	WP_025759084.1	lactonase family protein	-
CCL06_RS03080	622521..624431	+	1911	WP_025759086.1	BglG family transcription antiterminator	-
CCL06_RS03085	624509..624751	+	243	WP_025759087.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
CCL06_RS03090	624741..625025	+	285	WP_025759089.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CCL06_RS03095	625029..625493	-	465	WP_025759090.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
CCL06_RS03100	625599..627737	-	2139	WP_025759092.1	anaerobic ribonucleoside-triphosphate reductase	-
CCL06_RS03105	628114..629757	-	1644	WP_025759094.1	alpha,alpha-phosphotrehalase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-82)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-80)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10847.72 Da
• Isoelectric Point: 10.2746

>T77671 WP_025759089.1 NZ_CP021851:624741-625025 [Enterobacter cloacae]
MTYELVFDPRALKEWHKLGDTVKAQFKKKLAEVLAKPRNESARLHGLPDCYKIKLKASGYRLVYQVQDEVVKVVVIAIGK
REHSAVYHDANKRL

Nucleotide

• Length: 285 bp

>T77671 NZ_CP021851:624741-625025 [Enterobacter cloacae]
ATGACTTATGAACTGGTATTTGACCCAAGGGCGTTAAAGGAGTGGCATAAGCTGGGTGATACGGTGAAAGCGCAGTTTAA
GAAAAAACTTGCCGAGGTGCTGGCGAAACCCAGGAACGAATCAGCTCGTTTACACGGCCTGCCTGATTGTTACAAAATAA
AACTTAAAGCGTCCGGCTATCGCCTGGTCTATCAGGTTCAGGACGAAGTAGTGAAAGTCGTTGTTATTGCCATTGGGAAA
AGAGAACATTCGGCCGTTTATCATGACGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8958.34 Da
• Isoelectric Point: 5.0651

>AT77671 WP_025759087.1 NZ_CP021851:624509-624751 [Enterobacter cloacae]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAETGRMPVKTVTISDSEDALIQTVRERLANPQKGVKVSLDDL

Nucleotide

• Length: 243 bp

>AT77671 NZ_CP021851:624509-624751 [Enterobacter cloacae]
ATGGCCACGCTTAACGTCCGTCTGGATGACAAACTCAAAAACGAGGCTTACGCCGTGCTGGAAAAACTCAACATCACCCC
AACGGAAGCCGTGCGGTTACTGTTTCAATACGTTGCTGAAACGGGGCGCATGCCCGTGAAAACGGTCACGATTAGCGACA
GTGAAGATGCGCTGATACAGACGGTCCGGGAGCGACTGGCTAATCCGCAAAAGGGCGTTAAGGTTAGTCTGGATGACTTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A0F1BEH9