Detailed information of TA system TA077669

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 135617..136221
• Replicon: chromosome
• Accession: NZ_CP021851
• Organism: Enterobacter cloacae strain A1137

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A443XVE8
• Locus tag: CCL06_RS00675
• Protein ID: WP_047957473.1
• Coordinates: 135617..135802 (+)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CCL06_RS00680
• Protein ID: WP_025758405.1
• Coordinates: 135817..136221 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCL06_RS00660	132049..133587	+	1539	WP_025758409.1	aldehyde dehydrogenase AldB	-
CCL06_RS00665	133584..134546	-	963	WP_025758408.1	LysR family transcriptional regulator	-
CCL06_RS00670	134667..135407	+	741	WP_025758406.1	MipA/OmpV family protein	-
CCL06_RS00675	135617..135802	+	186	WP_047957473.1	type II toxin-antitoxin system HicA family toxin	Toxin
CCL06_RS00680	135817..136221	+	405	WP_025758405.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CCL06_RS00685	136277..138232	-	1956	WP_025758404.1	glycoside hydrolase family 127 protein	-
CCL06_RS00690	138243..139643	-	1401	WP_025758403.1	MFS transporter	-
CCL06_RS00695	139869..140687	+	819	WP_025758401.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6921.19 Da
• Isoelectric Point: 11.8224

>T77669 WP_047957473.1 NZ_CP021851:135617-135802 [Enterobacter cloacae]
VKSADVITVLIRHGWKCVRTKGSHHQFRHPVKKGLVTVPHPKKDIKPGTLAQIWRQAGIQH

Nucleotide

• Length: 186 bp

>T77669 NZ_CP021851:135617-135802 [Enterobacter cloacae]
GTGAAAAGTGCGGATGTCATTACTGTTCTGATCAGGCATGGCTGGAAATGTGTGAGAACGAAAGGAAGCCATCATCAGTT
TCGTCACCCCGTGAAGAAAGGGCTGGTGACTGTCCCACATCCTAAAAAGGATATTAAACCGGGCACCCTCGCGCAGATTT
GGCGGCAGGCGGGTATTCAACACTGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14976.85 Da
• Isoelectric Point: 4.3288

>AT77669 WP_025758405.1 NZ_CP021851:135817-136221 [Enterobacter cloacae]
MFYPAYIHADSDGSASGFFPDVPGCFFAGDSLDDAFQDARDALTAHFEALFEVDEVLPLPGNVEAHLERCPEDFIGGQWL
LVDINMKQFDGRAERINITMPRRLLVKIDSFVSEHPQFGNRSAFLAEAARRVLP

Nucleotide

• Length: 405 bp

>AT77669 NZ_CP021851:135817-136221 [Enterobacter cloacae]
ATGTTTTATCCGGCTTACATTCATGCTGATTCGGACGGAAGCGCCAGCGGTTTTTTCCCTGACGTTCCGGGCTGTTTTTT
TGCAGGTGATTCCCTGGATGACGCATTTCAGGATGCACGTGATGCACTCACCGCCCACTTCGAAGCGCTGTTTGAAGTGG
ATGAAGTACTGCCTCTTCCGGGGAATGTGGAAGCGCATCTGGAACGTTGTCCTGAGGATTTCATTGGTGGGCAATGGCTA
TTGGTGGATATTAATATGAAGCAGTTCGACGGCAGAGCCGAGCGTATCAACATCACGATGCCTCGACGTTTGCTGGTAAA
GATCGACTCCTTTGTAAGCGAGCACCCTCAGTTCGGCAATCGAAGCGCATTTCTGGCTGAGGCAGCACGTCGCGTGCTGC
CTTAA

Structures

Toxin

• AlphaFold DB: A0A443XVE8