Detailed information of TA system TA077667

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 50385..51047
• Replicon: chromosome
• Accession: NZ_CP021851
• Organism: Enterobacter cloacae strain A1137

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CCL06_RS00270
• Protein ID: WP_185857670.1
• Coordinates: 50649..51047 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0F1AKN0
• Locus tag: CCL06_RS00265
• Protein ID: WP_025757011.1
• Coordinates: 50385..50645 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCL06_RS00240	46455..47780	-	1326	WP_045286460.1	PTS sugar transporter subunit IIC	-
CCL06_RS00245	47792..48106	-	315	WP_014830072.1	PTS sugar transporter subunit IIB	-
CCL06_RS00250	48400..49350	+	951	WP_025757008.1	LacI family transcriptional regulator	-
CCL06_RS00255	49399..49695	-	297	WP_025757009.1	hypothetical protein	-
CCL06_RS00260	49836..50255	+	420	WP_025757010.1	GNAT family N-acetyltransferase	-
CCL06_RS00265	50385..50645	+	261	WP_025757011.1	virulence protein	Antitoxin
CCL06_RS00270	50649..51047	+	399	WP_185857670.1	type II toxin-antitoxin system VapC family toxin	Toxin
CCL06_RS00275	51169..51987	+	819	WP_176472031.1	lipoprotein NlpA	-
CCL06_RS00280	52084..53172	-	1089	WP_025757014.1	AAA family ATPase	-
CCL06_RS00285	53316..54275	-	960	WP_025757015.1	DUF523 and DUF1722 domain-containing protein	-
CCL06_RS00290	54378..54791	-	414	WP_025757016.1	hydroxyisourate hydrolase	-
CCL06_RS00295	54891..55616	-	726	WP_025757017.1	MerR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-121)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14808.99 Da
• Isoelectric Point: 5.9818

>T77667 WP_185857670.1 NZ_CP021851:50649-51047 [Enterobacter cloacae]
MLHMLDTNIVSHLVRQHPGVIKQYSRTPTEKMCISSVTEAELLYGIAKKQSDTLQKTILEFLKTITICDWDSEAAATYGE
LRAEMEKRGKVMGDLDQLIAAHAISRGTTIVTNDHAFRMVQELAVEDWTTAS

Nucleotide

• Length: 399 bp

>T77667 NZ_CP021851:50649-51047 [Enterobacter cloacae]
ATGCTGCATATGTTGGACACAAACATAGTAAGCCACCTCGTAAGGCAACATCCCGGGGTGATAAAACAATATTCTCGCAC
CCCAACAGAAAAAATGTGTATTTCAAGCGTGACGGAGGCCGAACTGCTTTATGGAATAGCCAAAAAGCAAAGTGACACAT
TACAAAAAACCATACTTGAGTTCCTTAAAACCATCACTATCTGCGACTGGGACAGTGAAGCCGCAGCAACGTACGGTGAA
CTCCGCGCAGAAATGGAAAAGAGAGGAAAAGTGATGGGCGATCTCGATCAGCTGATCGCTGCGCATGCTATTAGTCGGGG
TACAACGATAGTGACTAACGATCATGCGTTCAGAATGGTGCAGGAGCTTGCCGTCGAGGACTGGACGACAGCGTCGTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 10233.54 Da
• Isoelectric Point: 10.0877

>AT77667 WP_025757011.1 NZ_CP021851:50385-50645 [Enterobacter cloacae]
MERTAKLFRKGRNQAVMLPAEFTFDTKSVYIRRDEEGNVVLTARSEKERHRDNFLRLLKQTHVPDSFLSKEERNQSYTTR
DPFEGL

Nucleotide

• Length: 261 bp

>AT77667 NZ_CP021851:50385-50645 [Enterobacter cloacae]
ATGGAACGTACAGCAAAACTCTTCAGAAAGGGGCGAAATCAGGCAGTGATGCTGCCTGCTGAATTCACGTTTGATACAAA
GAGCGTATATATCCGACGAGATGAGGAGGGGAATGTCGTTTTGACTGCGAGATCGGAAAAGGAGCGTCACAGAGATAACT
TTTTACGTTTATTGAAGCAGACTCATGTTCCTGATTCATTTCTGAGCAAAGAGGAACGTAATCAGAGCTATACAACAAGA
GATCCATTTGAAGGGCTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F1AKN0