Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 84809..85410 Replicon plasmid pEC1515-2
Accession NZ_CP021846
Organism Escherichia coli strain EC1515

Toxin (Protein)


Gene name doc Uniprot ID -
Locus tag CDH89_RS27420 Protein ID WP_001216039.1
Coordinates 85030..85410 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID -
Locus tag CDH89_RS27415 Protein ID WP_001190711.1
Coordinates 84809..85030 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDH89_RS27365 79980..80303 + 324 WP_001175593.1 helix-turn-helix domain-containing protein -
CDH89_RS27375 80405..81102 + 698 Protein_84 IS1 family transposase -
CDH89_RS27395 82169..83431 + 1263 WP_069067570.1 hypothetical protein -
CDH89_RS27400 83504..83830 + 327 Protein_86 hypothetical protein -
CDH89_RS27405 83847..84544 - 698 Protein_87 IS1 family transposase -
CDH89_RS27415 84809..85030 + 222 WP_001190711.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CDH89_RS27420 85030..85410 + 381 WP_001216039.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
CDH89_RS27425 85415..85594 + 180 WP_000113018.1 hypothetical protein -
CDH89_RS27430 85622..86665 + 1044 WP_069067567.1 DUF968 domain-containing protein -
CDH89_RS27440 86968..87279 + 312 WP_001351993.1 phage antirepressor Ant -
CDH89_RS27445 87312..88163 - 852 WP_000611661.1 hypothetical protein -
CDH89_RS27450 88274..88483 - 210 WP_000874156.1 hypothetical protein -
CDH89_RS27465 89088..89309 + 222 WP_000542338.1 hypothetical protein -
CDH89_RS27470 89317..90348 + 1032 WP_000067537.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid qacE / aadA2 / dfrA12 / tet(A) - 1..92350 92350


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13558.27 Da        Isoelectric Point: 5.1514

>T77655 WP_001216039.1 NZ_CP021846:85030-85410 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRAALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T77655 NZ_CP021846:85030-85410 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTGATAGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCTGGCATGTC
AGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTGCCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8134.08 Da        Isoelectric Point: 4.5843

>AT77655 WP_001190711.1 NZ_CP021846:84809-85030 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVDR

Download         Length: 222 bp

>AT77655 NZ_CP021846:84809-85030 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCTCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTGACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References