Detailed information of TA system TA077647

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4533076..4533671
• Replicon: chromosome
• Accession: NZ_CP021844
• Organism: Escherichia coli strain EC1515

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: -
• Locus tag: CDH89_RS22870
• Protein ID: WP_088172274.1
• Coordinates: 4533076..4533426 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: U9Y2K1
• Locus tag: CDH89_RS22875
• Protein ID: WP_001223208.1
• Coordinates: 4533420..4533671 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDH89_RS22850	4528525..4529547	-	1023	WP_001362056.1	ABC transporter permease	-
CDH89_RS22855	4529561..4531063	-	1503	WP_044867109.1	sugar ABC transporter ATP-binding protein	-
CDH89_RS22860	4531203..4532159	-	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
CDH89_RS22865	4532469..4532999	+	531	WP_088172275.1	inorganic diphosphatase	-
CDH89_RS22870	4533076..4533426	-	351	WP_088172274.1	endoribonuclease toxin ChpB	Toxin
CDH89_RS22875	4533420..4533671	-	252	WP_001223208.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
CDH89_RS22880	4533883..4534224	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
CDH89_RS22885	4534227..4538006	-	3780	WP_000060913.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-115)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12522.43 Da
• Isoelectric Point: 5.5572

>T77647 WP_088172274.1 NZ_CP021844:c4533426-4533076 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLHTRLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T77647 NZ_CP021844:c4533426-4533076 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACACCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9271.58 Da
• Isoelectric Point: 4.4201

>AT77647 WP_001223208.1 NZ_CP021844:c4533671-4533420 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT77647 NZ_CP021844:c4533671-4533420 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LQ26