Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4285257..4285807 Replicon chromosome
Accession NZ_CP021844
Organism Escherichia coli strain EC1515

Toxin (Protein)


Gene name ccdB Uniprot ID Q32K46
Locus tag CDH89_RS21775 Protein ID WP_001160974.1
Coordinates 4285257..4285571 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag CDH89_RS21780 Protein ID WP_000125566.1
Coordinates 4285574..4285807 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDH89_RS21750 4280932..4282218 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
CDH89_RS21755 4282218..4283207 + 990 WP_000241272.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
CDH89_RS21760 4283204..4284025 + 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
CDH89_RS21765 4284028..4284405 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
CDH89_RS21770 4284412..4285260 + 849 WP_000257189.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
CDH89_RS21775 4285257..4285571 - 315 WP_001160974.1 CcdB family protein Toxin
CDH89_RS21780 4285574..4285807 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
CDH89_RS21785 4285893..4286372 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
CDH89_RS21790 4286564..4288426 - 1863 WP_000377191.1 glutathione-regulated potassium-efflux system protein KefC -
CDH89_RS21795 4288419..4288949 - 531 WP_000600729.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
CDH89_RS21800 4289060..4290391 - 1332 WP_001183198.1 MFS transporter -
CDH89_RS21805 4290449..4290736 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11737.84 Da        Isoelectric Point: 9.8517

>T77646 WP_001160974.1 NZ_CP021844:c4285571-4285257 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFIRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T77646 NZ_CP021844:c4285571-4285257 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTATCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT77646 WP_000125566.1 NZ_CP021844:c4285807-4285574 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT77646 NZ_CP021844:c4285807-4285574 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LRR3

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References