Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3961363..3962197 Replicon chromosome
Accession NZ_CP021844
Organism Escherichia coli strain EC1515

Toxin (Protein)


Gene name yeeV Uniprot ID A0A2X7CAN1
Locus tag CDH89_RS20170 Protein ID WP_039025883.1
Coordinates 3961363..3961740 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A2X5LBZ2
Locus tag CDH89_RS20175 Protein ID WP_039025882.1
Coordinates 3961829..3962197 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDH89_RS20145 3957067..3957966 + 900 WP_021577017.1 LysR family transcriptional regulator -
CDH89_RS20150 3958304..3959623 + 1320 WP_000144688.1 site-specific integrase -
CDH89_RS20155 3959716..3960564 - 849 WP_039025884.1 DUF4942 domain-containing protein -
CDH89_RS20160 3960661..3960858 - 198 WP_000772024.1 DUF957 domain-containing protein -
CDH89_RS20165 3960878..3961366 - 489 Protein_3711 hypothetical protein -
CDH89_RS20170 3961363..3961740 - 378 WP_039025883.1 type IV toxin-antitoxin system toxin CbtA Toxin
CDH89_RS20175 3961829..3962197 - 369 WP_039025882.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
CDH89_RS20180 3962247..3962801 - 555 WP_088172326.1 antitoxin of toxin-antitoxin stability system -
CDH89_RS20185 3962982..3964523 + 1542 WP_001298859.1 IS21-like element ISEc12 family transposase -
CDH89_RS20190 3964538..3965284 + 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -
CDH89_RS20195 3965492..3965713 - 222 WP_000692353.1 DUF987 domain-containing protein -
CDH89_RS20200 3965776..3966252 - 477 WP_001186774.1 RadC family protein -
CDH89_RS20205 3966268..3966753 - 486 WP_001563926.1 antirestriction protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island ant(3'')-Ia / blaTEM-1B / tet(M) fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE 3909121..4014966 105845


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14176.22 Da        Isoelectric Point: 7.8523

>T77644 WP_039025883.1 NZ_CP021844:c3961740-3961363 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQLEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYQEAKR

Download         Length: 378 bp

>T77644 NZ_CP021844:c3961740-3961363 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCCGGCATTTCACTGTGCGATGCAGTGAACTTTCTTGTTGAAAAATACGCGCTGGTGCGTACCGACCAGCTGGAATTC
AGTGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGAGCTCGCCGGGCGACTGGCCTGATGACCCGCGA
CAATTACCGAACGGTAAATAACATTACCCTGGGTAAGTATCAGGAGGCGAAACGATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13792.52 Da        Isoelectric Point: 5.9582

>AT77644 WP_039025882.1 NZ_CP021844:c3962197-3961829 [Escherichia coli]
VSDTFHETNYPDNNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT77644 NZ_CP021844:c3962197-3961829 [Escherichia coli]
GTGTCAGACACGTTCCATGAAACAAATTATCCCGACAACAACAACGATCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCTTTCCACTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2X7CAN1

Antitoxin

Source ID Structure
AlphaFold DB A0A2X5LBZ2

References