Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3736809..3737427 Replicon chromosome
Accession NZ_CP021844
Organism Escherichia coli strain EC1515

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CDH89_RS19050 Protein ID WP_001291435.1
Coordinates 3737209..3737427 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CDH89_RS19045 Protein ID WP_000344800.1
Coordinates 3736809..3737183 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDH89_RS19035 3731898..3733091 + 1194 WP_021577090.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CDH89_RS19040 3733114..3736263 + 3150 WP_021577089.1 multidrug efflux RND transporter permease subunit -
CDH89_RS19045 3736809..3737183 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CDH89_RS19050 3737209..3737427 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
CDH89_RS19055 3737599..3738150 + 552 WP_000102564.1 maltose O-acetyltransferase -
CDH89_RS19060 3738266..3738736 + 471 WP_000136192.1 YlaC family protein -
CDH89_RS19065 3738900..3740450 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CDH89_RS19070 3740492..3740845 - 354 WP_000878140.1 DUF1428 domain-containing protein -
CDH89_RS19080 3741224..3741535 + 312 WP_000409911.1 MGMT family protein -
CDH89_RS19085 3741566..3742138 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T77643 WP_001291435.1 NZ_CP021844:3737209-3737427 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T77643 NZ_CP021844:3737209-3737427 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT77643 WP_000344800.1 NZ_CP021844:3736809-3737183 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT77643 NZ_CP021844:3736809-3737183 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References