Detailed information of TA system TA077642

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3291747..3292452
• Replicon: chromosome
• Accession: NZ_CP021844
• Organism: Escherichia coli strain EC1515

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A246P0D5
• Locus tag: CDH89_RS16795
• Protein ID: WP_000539522.1
• Coordinates: 3291747..3292133 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CDH89_RS16800
• Protein ID: WP_001280945.1
• Coordinates: 3292123..3292452 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDH89_RS16775	3287751..3288377	+	627	WP_001295292.1	glutathione S-transferase GstB	-
CDH89_RS16780	3288374..3289489	-	1116	WP_021577174.1	aldose sugar dehydrogenase YliI	-
CDH89_RS16785	3289600..3289983	-	384	WP_000497137.1	biofilm formation regulator BssR	-
CDH89_RS16790	3290196..3291521	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
CDH89_RS16795	3291747..3292133	+	387	WP_000539522.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDH89_RS16800	3292123..3292452	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
CDH89_RS16805	3292522..3293850	-	1329	WP_088172359.1	GGDEF domain-containing protein	-
CDH89_RS16810	3293858..3296206	-	2349	WP_000950335.1	EAL domain-containing protein	-
CDH89_RS16815	3296384..3297295	-	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14292.51 Da
• Isoelectric Point: 10.2120

>T77642 WP_000539522.1 NZ_CP021844:3291747-3292133 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIKDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T77642 NZ_CP021844:3291747-3292133 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAAAGGATTTGATCGAAACCGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT77642 WP_001280945.1 NZ_CP021844:3292123-3292452 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT77642 NZ_CP021844:3292123-3292452 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A246P0D5