Detailed information of TA system TA077641

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2667551..2668189
• Replicon: chromosome
• Accession: NZ_CP021844
• Organism: Escherichia coli strain EC1515

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A246NT23
• Locus tag: CDH89_RS13675
• Protein ID: WP_021577430.1
• Coordinates: 2668013..2668189 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CDH89_RS13670
• Protein ID: WP_001270286.1
• Coordinates: 2667551..2667967 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDH89_RS13650	2662703..2663644	-	942	WP_021577433.1	ABC transporter permease	-
CDH89_RS13655	2663645..2664658	-	1014	WP_021577432.1	ABC transporter ATP-binding protein	-
CDH89_RS13660	2664676..2665821	-	1146	WP_021577431.1	ABC transporter substrate-binding protein	-
CDH89_RS13665	2666066..2667472	-	1407	WP_000760665.1	PLP-dependent aminotransferase family protein	-
CDH89_RS13670	2667551..2667967	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
CDH89_RS13675	2668013..2668189	-	177	WP_021577430.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
CDH89_RS13680	2668411..2668641	+	231	WP_000494244.1	YncJ family protein	-
CDH89_RS13685	2668733..2670694	-	1962	WP_021577429.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
CDH89_RS13690	2670767..2671303	-	537	WP_021577428.1	DNA-binding transcriptional regulator SutR	-
CDH89_RS13695	2671356..2672570	+	1215	WP_071780838.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2672610..2673758	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6692.77 Da
• Isoelectric Point: 10.9223

>T77641 WP_021577430.1 NZ_CP021844:c2668189-2668013 [Escherichia coli]
VKQSEFRRWLESQGVDVANVSNHLKLGFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T77641 NZ_CP021844:c2668189-2668013 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGTCAGCAACCATTTGAAACTTGG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT77641 WP_001270286.1 NZ_CP021844:c2667967-2667551 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT77641 NZ_CP021844:c2667967-2667551 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCACGATCATTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTGA

Structures

Toxin

• AlphaFold DB: A0A246NT23