Detailed information of TA system TA077603

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3937839..3938477
• Replicon: chromosome
• Accession: NZ_CP021840
• Organism: Escherichia coli strain EC974

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A246NT23
• Locus tag: CDH88_RS19680
• Protein ID: WP_021577430.1
• Coordinates: 3937839..3938015 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CDH88_RS19685
• Protein ID: WP_001270286.1
• Coordinates: 3938061..3938477 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDH88_RS19660	3933458..3934672	-	1215	WP_071780838.1	BenE family transporter YdcO	-
CDH88_RS19665	3934725..3935261	+	537	WP_021577428.1	DNA-binding transcriptional regulator SutR	-
CDH88_RS19670	3935334..3937295	+	1962	WP_021577429.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
CDH88_RS19675	3937387..3937617	-	231	WP_000494244.1	YncJ family protein	-
CDH88_RS19680	3937839..3938015	+	177	WP_021577430.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
CDH88_RS19685	3938061..3938477	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
CDH88_RS19690	3938556..3939962	+	1407	WP_000760665.1	PLP-dependent aminotransferase family protein	-
CDH88_RS19695	3940207..3941352	+	1146	WP_021577431.1	ABC transporter substrate-binding protein	-
CDH88_RS19700	3941370..3942383	+	1014	WP_021577432.1	ABC transporter ATP-binding protein	-
CDH88_RS19705	3942384..3943325	+	942	WP_021577433.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3932270..3933418	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6692.77 Da
• Isoelectric Point: 10.9223

>T77603 WP_021577430.1 NZ_CP021840:3937839-3938015 [Escherichia coli]
VKQSEFRRWLESQGVDVANVSNHLKLGFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T77603 NZ_CP021840:3937839-3938015 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGTCAGCAACCATTTGAAACTTGG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT77603 WP_001270286.1 NZ_CP021840:3938061-3938477 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT77603 NZ_CP021840:3938061-3938477 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCACGATCATTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTGA

Structures

Toxin

• AlphaFold DB: A0A246NT23