Detailed information of TA system TA077602

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3313576..3314281
• Replicon: chromosome
• Accession: NZ_CP021840
• Organism: Escherichia coli strain EC974

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A246P0D5
• Locus tag: CDH88_RS16565
• Protein ID: WP_000539522.1
• Coordinates: 3313895..3314281 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CDH88_RS16560
• Protein ID: WP_001280945.1
• Coordinates: 3313576..3313905 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDH88_RS16545	3308733..3309644	+	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-
CDH88_RS16550	3309822..3312170	+	2349	WP_000950335.1	EAL domain-containing protein	-
CDH88_RS16555	3312178..3313506	+	1329	WP_088172359.1	GGDEF domain-containing protein	-
CDH88_RS16560	3313576..3313905	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
CDH88_RS16565	3313895..3314281	-	387	WP_000539522.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDH88_RS16570	3314507..3315832	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
CDH88_RS16575	3316045..3316428	+	384	WP_000497137.1	biofilm formation regulator BssR	-
CDH88_RS16580	3316539..3317654	+	1116	WP_021577174.1	aldose sugar dehydrogenase YliI	-
CDH88_RS16585	3317651..3318277	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14292.51 Da
• Isoelectric Point: 10.2120

>T77602 WP_000539522.1 NZ_CP021840:c3314281-3313895 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIKDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T77602 NZ_CP021840:c3314281-3313895 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAAAGGATTTGATCGAAACCGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT77602 WP_001280945.1 NZ_CP021840:c3313905-3313576 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT77602 NZ_CP021840:c3313905-3313576 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A246P0D5