Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2867628..2868246 Replicon chromosome
Accession NZ_CP021840
Organism Escherichia coli strain EC974

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CDH88_RS14305 Protein ID WP_001291435.1
Coordinates 2867628..2867846 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CDH88_RS14310 Protein ID WP_000344800.1
Coordinates 2867872..2868246 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDH88_RS14270 2862917..2863489 + 573 WP_000779831.1 YbaY family lipoprotein -
CDH88_RS14275 2863520..2863831 - 312 WP_000409911.1 MGMT family protein -
CDH88_RS14285 2864210..2864563 + 354 WP_000878140.1 DUF1428 domain-containing protein -
CDH88_RS14290 2864605..2866155 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CDH88_RS14295 2866319..2866789 - 471 WP_000136192.1 YlaC family protein -
CDH88_RS14300 2866905..2867456 - 552 WP_000102564.1 maltose O-acetyltransferase -
CDH88_RS14305 2867628..2867846 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
CDH88_RS14310 2867872..2868246 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CDH88_RS14315 2868792..2871941 - 3150 WP_021577089.1 multidrug efflux RND transporter permease subunit -
CDH88_RS14320 2871964..2873157 - 1194 WP_021577090.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T77601 WP_001291435.1 NZ_CP021840:c2867846-2867628 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T77601 NZ_CP021840:c2867846-2867628 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT77601 WP_000344800.1 NZ_CP021840:c2868246-2867872 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT77601 NZ_CP021840:c2868246-2867872 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References