Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2642858..2643692 Replicon chromosome
Accession NZ_CP021840
Organism Escherichia coli strain EC974

Toxin (Protein)


Gene name yeeV Uniprot ID A0A2X7CAN1
Locus tag CDH88_RS13190 Protein ID WP_039025883.1
Coordinates 2643315..2643692 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A2X5LBZ2
Locus tag CDH88_RS13185 Protein ID WP_039025882.1
Coordinates 2642858..2643226 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDH88_RS13155 2638302..2638787 + 486 WP_001563926.1 antirestriction protein -
CDH88_RS13160 2638803..2639279 + 477 WP_001186774.1 RadC family protein -
CDH88_RS13165 2639342..2639563 + 222 WP_000692353.1 DUF987 domain-containing protein -
CDH88_RS13170 2639771..2640517 - 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -
CDH88_RS13175 2640532..2642073 - 1542 WP_001298859.1 IS21-like element ISEc12 family transposase -
CDH88_RS13180 2642254..2642808 + 555 WP_088172326.1 antitoxin of toxin-antitoxin stability system -
CDH88_RS13185 2642858..2643226 + 369 WP_039025882.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
CDH88_RS13190 2643315..2643692 + 378 WP_039025883.1 type IV toxin-antitoxin system toxin CbtA Toxin
CDH88_RS13195 2643689..2644177 + 489 Protein_2416 hypothetical protein -
CDH88_RS13200 2644197..2644394 + 198 WP_000772024.1 DUF957 domain-containing protein -
CDH88_RS13205 2644491..2645339 + 849 WP_039025884.1 DUF4942 domain-containing protein -
CDH88_RS13210 2645432..2646751 - 1320 WP_000144688.1 site-specific integrase -
CDH88_RS13215 2647089..2647988 - 900 WP_021577017.1 LysR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island ant(3'')-Ia / tet(M) / blaTEM-1B yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC 2590089..2696988 106899


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14176.22 Da        Isoelectric Point: 7.8523

>T77600 WP_039025883.1 NZ_CP021840:2643315-2643692 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQLEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYQEAKR

Download         Length: 378 bp

>T77600 NZ_CP021840:2643315-2643692 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCCGGCATTTCACTGTGCGATGCAGTGAACTTTCTTGTTGAAAAATACGCGCTGGTGCGTACCGACCAGCTGGAATTC
AGTGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGAGCTCGCCGGGCGACTGGCCTGATGACCCGCGA
CAATTACCGAACGGTAAATAACATTACCCTGGGTAAGTATCAGGAGGCGAAACGATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13792.52 Da        Isoelectric Point: 5.9582

>AT77600 WP_039025882.1 NZ_CP021840:2642858-2643226 [Escherichia coli]
VSDTFHETNYPDNNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT77600 NZ_CP021840:2642858-2643226 [Escherichia coli]
GTGTCAGACACGTTCCATGAAACAAATTATCCCGACAACAACAACGATCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCTTTCCACTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2X7CAN1

Antitoxin

Source ID Structure
AlphaFold DB A0A2X5LBZ2

References