Detailed information of TA system TA077597

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 2071382..2071977
• Replicon: chromosome
• Accession: NZ_CP021840
• Organism: Escherichia coli strain EC974

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: -
• Locus tag: CDH88_RS10490
• Protein ID: WP_088172274.1
• Coordinates: 2071627..2071977 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: U9Y2K1
• Locus tag: CDH88_RS10485
• Protein ID: WP_001223208.1
• Coordinates: 2071382..2071633 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDH88_RS10475	2067047..2070826	+	3780	WP_000060913.1	autotransporter assembly complex protein TamB	-
CDH88_RS10480	2070829..2071170	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
CDH88_RS10485	2071382..2071633	+	252	WP_001223208.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
CDH88_RS10490	2071627..2071977	+	351	WP_088172274.1	endoribonuclease toxin ChpB	Toxin
CDH88_RS10495	2072054..2072584	-	531	WP_088172275.1	inorganic diphosphatase	-
CDH88_RS10500	2072894..2073850	+	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
CDH88_RS10505	2073990..2075492	+	1503	WP_044867109.1	sugar ABC transporter ATP-binding protein	-
CDH88_RS10510	2075506..2076528	+	1023	WP_001362056.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-115)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12522.43 Da
• Isoelectric Point: 5.5572

>T77597 WP_088172274.1 NZ_CP021840:2071627-2071977 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLHTRLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T77597 NZ_CP021840:2071627-2071977 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACACCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9271.58 Da
• Isoelectric Point: 4.4201

>AT77597 WP_001223208.1 NZ_CP021840:2071382-2071633 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT77597 NZ_CP021840:2071382-2071633 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LQ26