Detailed information of TA system TA077594

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1122389..1123020
• Replicon: chromosome
• Accession: NZ_CP021840
• Organism: Escherichia coli strain EC974

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: CDH88_RS05855
• Protein ID: WP_088172239.1
• Coordinates: 1122745..1123020 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: U9Y7E1
• Locus tag: CDH88_RS05850
• Protein ID: WP_000593555.1
• Coordinates: 1122389..1122748 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDH88_RS05820	1117501..1117974	+	474	WP_047669097.1	PTS sugar transporter subunit IIA	-
CDH88_RS05825	1117971..1118252	+	282	WP_000084022.1	PTS sugar transporter subunit IIB	-
CDH88_RS05830	1118329..1119687	+	1359	WP_001302220.1	PTS galactitol transporter subunit IIC	-
CDH88_RS05835	1119680..1121188	+	1509	WP_088172238.1	carbohydrate kinase	-
CDH88_RS05840	1121178..1121447	+	270	WP_000933060.1	HPr family phosphocarrier protein	-
CDH88_RS05845	1121479..1122339	+	861	WP_001131788.1	class II aldolase	-
CDH88_RS05850	1122389..1122748	-	360	WP_000593555.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CDH88_RS05855	1122745..1123020	-	276	WP_088172239.1	type II toxin-antitoxin system HicA family toxin	Toxin
CDH88_RS05860	1123093..1124217	-	1125	WP_001314210.1	ABC transporter permease	-
CDH88_RS05865	1124217..1126952	-	2736	WP_000149132.1	ribosome-associated ATPase/putative transporter RbbA	-
CDH88_RS05870	1126949..1128016	-	1068	WP_000361517.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1116730..1141547	24817

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10192.95 Da
• Isoelectric Point: 10.7076

>T77594 WP_088172239.1 NZ_CP021840:c1123020-1122745 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKIPVPQGIKWSDIESQVKALGGEIKEGRGSRCKLILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T77594 NZ_CP021840:c1123020-1122745 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAATTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACAGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGC
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13402.27 Da
• Isoelectric Point: 5.1987

>AT77594 WP_000593555.1 NZ_CP021840:c1122748-1122389 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT77594 NZ_CP021840:c1122748-1122389 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAGCTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LKZ6