Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 480815..481469 Replicon chromosome
Accession NZ_CP021840
Organism Escherichia coli strain EC974

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag CDH88_RS02485 Protein ID WP_000244777.1
Coordinates 480815..481222 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A7B3IGV2
Locus tag CDH88_RS02490 Protein ID WP_021577895.1
Coordinates 481203..481469 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDH88_RS02465 476772..478505 - 1734 WP_000813188.1 single-stranded-DNA-specific exonuclease RecJ -
CDH88_RS02470 478511..479221 - 711 WP_000715210.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CDH88_RS02475 479246..480142 - 897 WP_000806630.1 site-specific tyrosine recombinase XerD -
CDH88_RS02480 480254..480775 + 522 WP_001055874.1 flavodoxin FldB -
CDH88_RS02485 480815..481222 - 408 WP_000244777.1 toxin CptA Toxin
CDH88_RS02490 481203..481469 - 267 WP_021577895.1 FAD assembly factor SdhE Antitoxin
CDH88_RS02495 481712..482692 + 981 WP_021577896.1 tRNA-modifying protein YgfZ -
CDH88_RS02500 482769..483428 - 660 WP_000250270.1 hemolysin III family protein -
CDH88_RS02505 483592..483903 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
CDH88_RS02510 483948..485381 + 1434 WP_021577897.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T77589 WP_000244777.1 NZ_CP021840:c481222-480815 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T77589 NZ_CP021840:c481222-480815 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10616.23 Da        Isoelectric Point: 5.6483

>AT77589 WP_021577895.1 NZ_CP021840:c481469-481203 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDRLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT77589 NZ_CP021840:c481469-481203 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGATTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTTGAATGTGACGATCCAGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
AlphaFold DB A0A7B3IGV2

References