Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 335222..335805 Replicon chromosome
Accession NZ_CP021840
Organism Escherichia coli strain EC974

Toxin (Protein)


Gene name mazF Uniprot ID U9Y5H3
Locus tag CDH88_RS01810 Protein ID WP_000254735.1
Coordinates 335222..335557 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A246NW33
Locus tag CDH88_RS01815 Protein ID WP_021577864.1
Coordinates 335557..335805 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDH88_RS01795 331108..332406 - 1299 WP_000036723.1 phosphopyruvate hydratase -
CDH88_RS01800 332494..334131 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
CDH88_RS01805 334359..335150 - 792 WP_021577863.1 nucleoside triphosphate pyrophosphohydrolase -
CDH88_RS01810 335222..335557 - 336 WP_000254735.1 endoribonuclease MazF Toxin
CDH88_RS01815 335557..335805 - 249 WP_021577864.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
CDH88_RS01820 335883..338117 - 2235 WP_000226815.1 GTP pyrophosphokinase -
CDH88_RS01825 338165..339466 - 1302 WP_021577865.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12070.03 Da        Isoelectric Point: 8.2605

>T77588 WP_000254735.1 NZ_CP021840:c335557-335222 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRAKGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T77588 NZ_CP021840:c335557-335222 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAAAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9369.76 Da        Isoelectric Point: 4.4665

>AT77588 WP_021577864.1 NZ_CP021840:c335805-335557 [Escherichia coli]
MIHSSIKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT77588 NZ_CP021840:c335805-335557 [Escherichia coli]
ATGATCCACAGTAGCATAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9Y5H3

Antitoxin

Source ID Structure
AlphaFold DB A0A246NW33

References