Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2369179..2369873 Replicon chromosome
Accession NZ_CP021838
Organism Anoxybacillus flavithermus strain 52-1A

Toxin (Protein)


Gene name mazF Uniprot ID A0A178T4V4
Locus tag CA592_RS12720 Protein ID WP_035018420.1
Coordinates 2369179..2369565 (-) Length 129 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A1Z2V958
Locus tag CA592_RS12725 Protein ID WP_004888884.1
Coordinates 2369565..2369873 (-) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA592_RS12695 2364640..2364945 + 306 Protein_2441 ATP-binding cassette domain-containing protein -
CA592_RS12700 2364958..2366091 - 1134 WP_088223289.1 IS4 family transposase -
CA592_RS12705 2366287..2366720 + 434 Protein_2443 ABC-2 transporter permease -
CA592_RS12710 2366849..2368507 - 1659 WP_088223227.1 IS1634 family transposase -
CA592_RS15435 2368922..2369071 + 150 WP_163151105.1 hypothetical protein -
CA592_RS12720 2369179..2369565 - 387 WP_035018420.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CA592_RS12725 2369565..2369873 - 309 WP_004888884.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CA592_RS15265 2370460..2370525 + 66 WP_128356962.1 KxYKxGKxW signal peptide domain-containing protein -
CA592_RS12730 2371175..2371441 + 267 Protein_2449 helix-turn-helix domain-containing protein -
CA592_RS12735 2371765..2373462 + 1698 WP_088223644.1 hypothetical protein -
CA592_RS12740 2373582..2374073 + 492 WP_128356957.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2348692..2375355 26663
- flank IS/Tn - - 2364958..2366091 1133


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-119)

Antitoxin

Antitoxin PemI-like

(23-100)


Sequences

Toxin        


Download         Length: 129 a.a.        Molecular weight: 14347.34 Da        Isoelectric Point: 4.6190

>T77587 WP_035018420.1 NZ_CP021838:c2369565-2369179 [Anoxybacillus flavithermus]
MPADVPERGDFVVLNFNPQTGHEQAGRRNAIVLSPKEFNQATGFIAVCPITNQKKGYPFEVDLPKEGISLDGDGYPITGV
VLTDQIKSLDWRARNLKILKKYNPEDAQIEEIDKIIDECLAKIETYLT

Download         Length: 387 bp

>T77587 NZ_CP021838:c2369565-2369179 [Anoxybacillus flavithermus]
ATGCCAGCAGACGTACCAGAGAGAGGCGATTTCGTTGTTTTAAACTTCAATCCACAAACTGGTCATGAACAGGCTGGGAG
AAGGAATGCTATTGTTTTATCGCCTAAAGAATTCAATCAAGCAACAGGATTTATAGCTGTTTGTCCGATTACCAATCAAA
AGAAGGGTTACCCTTTCGAAGTGGATTTACCAAAAGAAGGAATATCCCTTGATGGCGATGGCTATCCAATAACAGGGGTA
GTTTTAACTGATCAAATTAAATCATTGGATTGGAGAGCTCGTAATCTAAAAATTTTAAAGAAGTATAATCCAGAAGATGC
ACAAATTGAAGAAATAGATAAAATCATTGATGAATGCCTAGCGAAAATAGAAACATATCTGACTTGA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11662.38 Da        Isoelectric Point: 7.0153

>AT77587 WP_004888884.1 NZ_CP021838:c2369873-2369565 [Anoxybacillus flavithermus]
MYSKEYLENKINQSKGVDYMSTVIVQKWGNSLGIRIPKDAADKIGIKQGSEIELNVIGNEGIITLKPKRTRKKYTLEELI
SQITPENRHEEIDFGIEGRELI

Download         Length: 309 bp

>AT77587 NZ_CP021838:c2369873-2369565 [Anoxybacillus flavithermus]
ATGTATTCAAAAGAATATTTAGAAAACAAAATAAACCAGAGTAAAGGGGTTGACTACATGTCTACAGTTATTGTTCAAAA
ATGGGGAAACAGTTTGGGCATTCGAATCCCAAAAGATGCTGCTGATAAAATAGGGATTAAACAAGGTTCCGAAATAGAAT
TAAATGTGATTGGAAATGAAGGTATCATCACATTAAAGCCAAAAAGAACACGGAAGAAATACACACTGGAAGAACTAATA
TCACAGATTACACCTGAAAATCGACATGAGGAAATTGATTTCGGGATAGAAGGGCGTGAATTGATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A178T4V4

Antitoxin

Source ID Structure
AlphaFold DB A0A1Z2V958

References