Detailed information of TA system TA077584

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 109044..109780
• Replicon: plasmid tig00000500_pilon
• Accession: NZ_CP021834
• Organism: Klebsiella pneumoniae strain AR_0120

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: AM367_RS28435
• Protein ID: WP_003026803.1
• Coordinates: 109298..109780 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: AM367_RS28430
• Protein ID: WP_003026799.1
• Coordinates: 109044..109310 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM367_RS28385	105106..105468	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
AM367_RS28390	105518..105868	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
AM367_RS28395	106226..106495	+	270	WP_004152102.1	hypothetical protein	-
AM367_RS28400	106483..107058	+	576	WP_004152103.1	hypothetical protein	-
AM367_RS28405	107089..107583	+	495	WP_004152104.1	DNA-binding protein	-
AM367_RS28410	107627..107995	+	369	WP_004152105.1	hypothetical protein	-
AM367_RS28415	108029..108232	+	204	WP_004152106.1	hemolysin expression modulator Hha	-
AM367_RS28420	108281..108538	+	258	WP_004152107.1	hypothetical protein	-
AM367_RS28425	108614..108868	+	255	WP_004152108.1	hypothetical protein	-
AM367_RS28430	109044..109310	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
AM367_RS28435	109298..109780	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
AM367_RS28440	109992..111338	+	1347	WP_020314316.1	ISNCY family transposase	-
AM367_RS28445	111839..113093	-	1255	Protein_125	IS3 family transposase	-
AM367_RS28450	113181..114143	-	963	WP_004152113.1	M48 family metalloprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-Ia / mph(A) / sul1 / qacE / aadA2 / dfrA12	-	1..212837	212837

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T77584 WP_003026803.1 NZ_CP021834:109298-109780 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T77584 NZ_CP021834:109298-109780 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT77584 WP_003026799.1 NZ_CP021834:109044-109310 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT77584 NZ_CP021834:109044-109310 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66