Detailed information of TA system TA077565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 1490283..1490950
• Replicon: plasmid psymB
• Accession: NZ_CP021831
• Organism: Sinorhizobium meliloti strain HM006

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CDO22_RS33340
• Protein ID: WP_017273388.1
• Coordinates: 1490507..1490950 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q92WE1
• Locus tag: CDO22_RS33335
• Protein ID: WP_010975287.1
• Coordinates: 1490283..1490510 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO22_RS33310	1485676..1486557	-	882	WP_003525869.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
CDO22_RS33315	1486675..1487967	-	1293	WP_010975290.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
CDO22_RS33325	1488336..1489625	+	1290	WP_017273387.1	SDR family oxidoreductase	-
CDO22_RS33330	1489625..1490098	+	474	WP_015008545.1	DUF2269 family protein	-
CDO22_RS33335	1490283..1490510	+	228	WP_010975287.1	hypothetical protein	Antitoxin
CDO22_RS33340	1490507..1490950	+	444	WP_017273388.1	PIN domain-containing protein	Toxin
CDO22_RS33345	1490966..1491904	-	939	WP_040121074.1	choline ABC transporter substrate-binding protein	-
CDO22_RS33350	1491973..1492803	-	831	WP_014527489.1	SDR family oxidoreductase	-
CDO22_RS33355	1492800..1493297	-	498	WP_010975282.1	acetone carboxylase subunit gamma	-
CDO22_RS33360	1493301..1495493	-	2193	WP_040121075.1	hydantoinase B/oxoprolinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	htpB	1..1628141	1628141

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 15619.98 Da
• Isoelectric Point: 7.4215

>T77565 WP_017273388.1 NZ_CP021831:1490507-1490950 [Sinorhizobium meliloti]
VTFLLDVNVLIALIDPSHIGHDDAHEWFASIGQTAWATCPITENGVIRIVGNPKYPNSPGSPLPVMEIVAKLRSLPGHVF
WPDDVSLVGSSDIIPSKILTSGQVTDTYLLALAKARGGKLATFDRKLSAAAVTKGNSALHLIATNRS

Nucleotide

• Length: 444 bp

>T77565 NZ_CP021831:1490507-1490950 [Sinorhizobium meliloti]
GTGACTTTCCTGCTCGACGTCAATGTGCTGATTGCCTTGATCGATCCGAGCCATATAGGCCATGATGATGCGCACGAATG
GTTCGCGTCGATTGGTCAGACCGCGTGGGCCACCTGCCCAATTACCGAAAATGGCGTCATCCGGATCGTCGGCAATCCCA
AATATCCCAATTCTCCCGGCTCACCGTTACCCGTGATGGAGATCGTCGCCAAATTGCGGTCGCTCCCCGGTCATGTTTTT
TGGCCGGACGATGTGAGCCTTGTCGGGTCAAGTGATATCATCCCATCGAAAATCCTGACTTCGGGGCAGGTGACTGACAC
CTATCTGCTTGCGCTCGCCAAGGCGCGTGGGGGCAAGCTGGCGACATTCGATCGCAAGCTGTCGGCAGCGGCGGTCACGA
AGGGCAATTCCGCCTTGCACCTGATCGCGACGAATAGGTCGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8174.39 Da
• Isoelectric Point: 9.1096

>AT77565 WP_010975287.1 NZ_CP021831:1490283-1490510 [Sinorhizobium meliloti]
MRTTLAIDDDVLIAAKAMATQQQRSVGEVISELARRSLRRPRSGGERNGIPLLSPRPDAPPVTLEIVNALRDELA

Nucleotide

• Length: 228 bp

>AT77565 NZ_CP021831:1490283-1490510 [Sinorhizobium meliloti]
ATGAGAACGACCTTGGCTATTGACGACGATGTCCTGATCGCCGCAAAGGCAATGGCGACCCAACAGCAGCGGAGCGTCGG
CGAGGTAATTTCCGAACTGGCGAGGCGTTCTCTGCGCCGGCCGCGCAGCGGCGGCGAGCGTAATGGAATTCCTCTCCTGT
CGCCACGTCCGGACGCACCGCCCGTGACGCTCGAGATCGTCAACGCCCTGCGTGACGAACTGGCGTGA

Structures

Antitoxin

• AlphaFold DB: Q92WE1