Detailed information of TA system TA077564

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 557414..557969
• Replicon: plasmid psymB
• Accession: NZ_CP021831
• Organism: Sinorhizobium meliloti strain HM006

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: CDO22_RS28815
• Protein ID: WP_041169867.1
• Coordinates: 557652..557969 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: H0FXK0
• Locus tag: CDO22_RS28810
• Protein ID: WP_003527850.1
• Coordinates: 557414..557662 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO22_RS28790	553651..554982	+	1332	WP_040120960.1	O-antigen ligase family protein	-
CDO22_RS28795	554975..555730	+	756	WP_015242828.1	polysaccharide deacetylase family protein	-
CDO22_RS28800	555741..556970	+	1230	WP_015242829.1	glycosyltransferase family 4 protein	-
CDO22_RS28805	557087..557314	+	228	WP_015242830.1	hypothetical protein	-
CDO22_RS28810	557414..557662	+	249	WP_003527850.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
CDO22_RS28815	557652..557969	+	318	WP_041169867.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDO22_RS28820	557940..558140	-	201	Protein_521	helix-turn-helix domain-containing protein	-
CDO22_RS28825	558462..559631	+	1170	WP_015242832.1	glycosyltransferase family 4 protein	-
CDO22_RS28830	559628..560017	+	390	WP_015242833.1	hypothetical protein	-
CDO22_RS28835	560048..561091	+	1044	WP_015242834.1	glycosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	htpB	1..1628141	1628141

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-93)

Antitoxin

• Antitoxin ParD (3-78)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12255.06 Da
• Isoelectric Point: 11.0222

>T77564 WP_041169867.1 NZ_CP021831:557652-557969 [Sinorhizobium meliloti]
MATERPFRLAPAAKADLRKIWRHTARRWSLEQAETYQDQLYTAFEGLAAGTKKGRNVDVRPGYLKYPAGAHIVYFRDRGD
RIDIIRILHGRMDAQRTHRAYMRAP

Nucleotide

• Length: 318 bp

>T77564 NZ_CP021831:557652-557969 [Sinorhizobium meliloti]
ATGGCGACTGAACGGCCTTTCCGGCTCGCTCCTGCCGCCAAGGCCGACCTTCGGAAGATATGGCGCCACACGGCTCGCCG
CTGGTCTCTGGAACAGGCGGAGACCTACCAAGACCAGCTTTATACTGCATTTGAGGGGCTGGCCGCAGGGACGAAGAAAG
GCCGCAATGTCGATGTGCGGCCGGGTTATCTCAAATACCCGGCAGGAGCGCACATCGTCTATTTTCGGGATCGCGGCGAC
CGGATCGACATCATCCGCATCCTGCATGGCCGGATGGACGCCCAACGAACGCATCGGGCTTATATGCGGGCGCCGTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8888.73 Da
• Isoelectric Point: 4.2176

>AT77564 WP_003527850.1 NZ_CP021831:557414-557662 [Sinorhizobium meliloti]
MAARSTSISLGDHFAGFIDSQVQTGRYGSASDVVRAGLRLLEEHEAKVRALEAALIEGEESGEPEPFDNEAFKAEMRAEY
GD

Nucleotide

• Length: 249 bp

>AT77564 NZ_CP021831:557414-557662 [Sinorhizobium meliloti]
ATGGCTGCTAGAAGCACATCTATTTCCCTTGGCGATCACTTCGCCGGTTTCATCGACAGCCAGGTGCAAACCGGCCGCTA
CGGCTCCGCCAGCGACGTTGTGCGGGCTGGACTTCGGCTTTTGGAAGAACACGAGGCGAAGGTAAGGGCGCTGGAAGCGG
CTTTGATCGAGGGCGAAGAATCCGGCGAGCCAGAGCCATTCGACAACGAAGCGTTCAAAGCCGAAATGCGCGCCGAATAT
GGCGACTGA

Structures

Antitoxin

• AlphaFold DB: H0FXK0