Detailed information of TA system TA077563

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 235427..235961
• Replicon: plasmid psymB
• Accession: NZ_CP021831
• Organism: Sinorhizobium meliloti strain HM006

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: CDO22_RS36025
• Protein ID: WP_017268214.1
• Coordinates: 235427..235723 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: H0FXK0
• Locus tag: CDO22_RS27315
• Protein ID: WP_003527850.1
• Coordinates: 235713..235961 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO22_RS27285	230902..232281	-	1380	WP_027988292.1	sodium:alanine symporter family protein	-
CDO22_RS27290	232815..233024	-	210	WP_003527838.1	dodecin family protein	-
CDO22_RS27295	233314..233760	+	447	WP_014527651.1	DNA-binding protein	-
CDO22_RS27300	233764..233949	-	186	WP_014527652.1	hypothetical protein	-
CDO22_RS27305	234116..234514	-	399	WP_010974988.1	hypothetical protein	-
CDO22_RS36020	235088..235348	+	261	Protein_229	helix-turn-helix domain-containing protein	-
CDO22_RS36025	235427..235723	-	297	WP_017268214.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDO22_RS27315	235713..235961	-	249	WP_003527850.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
CDO22_RS27330	236580..236705	+	126	Protein_232	IS481 family transposase	-
CDO22_RS27335	236789..237583	-	795	WP_014527654.1	methyltransferase domain-containing protein	-
CDO22_RS27340	237587..238387	-	801	WP_014527655.1	ABC transporter ATP-binding protein	-
CDO22_RS27345	238384..239427	-	1044	WP_010974980.1	iron ABC transporter permease	-
CDO22_RS27350	239424..240491	-	1068	WP_027988288.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	htpB	1..1628141	1628141
	-	flank	IS/Tn	-	-	236514..236705	191

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-93)

Antitoxin

• Antitoxin ParD (3-78)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11490.20 Da
• Isoelectric Point: 10.7543

>T77563 WP_017268214.1 NZ_CP021831:c235723-235427 [Sinorhizobium meliloti]
MATERPFRLAPAAKADLRKIWRYKARRWSLEQAETYQDQLYTAFEGLAAGTKKGRNVDVRPGYLKYPAGSHIVYFRDRGD
RIDIIRILHGRMDAQRHL

Nucleotide

• Length: 297 bp

>T77563 NZ_CP021831:c235723-235427 [Sinorhizobium meliloti]
ATGGCGACTGAACGGCCTTTCCGGCTCGCTCCTGCCGCCAAGGCCGACCTTCGGAAGATATGGCGCTACAAGGCTCGCCG
CTGGTCTCTGGAACAGGCGGAGACCTACCAAGACCAGCTTTATACTGCATTTGAGGGACTGGCCGCAGGGACGAAGAAAG
GCCGCAATGTCGATGTGCGACCGGGTTATCTCAAATACCCGGCAGGATCGCACATCGTCTATTTTCGGGATCGCGGCGAC
CGGATCGACATCATCCGCATCCTGCATGGCCGGATGGATGCCCAACGGCATTTGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8888.73 Da
• Isoelectric Point: 4.2176

>AT77563 WP_003527850.1 NZ_CP021831:c235961-235713 [Sinorhizobium meliloti]
MAARSTSISLGDHFAGFIDSQVQTGRYGSASDVVRAGLRLLEEHEAKVRALEAALIEGEESGEPEPFDNEAFKAEMRAEY
GD

Nucleotide

• Length: 249 bp

>AT77563 NZ_CP021831:c235961-235713 [Sinorhizobium meliloti]
ATGGCTGCTAGAAGCACATCTATTTCCCTTGGCGATCACTTCGCCGGTTTCATCGACAGCCAGGTGCAAACCGGCCGCTA
CGGCTCCGCCAGCGACGTTGTGCGGGCTGGACTTCGGCTTTTGGAAGAACACGAGGCGAAGGTAAGGGCGCTGGAAGCGG
CTTTGATCGAGGGCGAAGAATCCGGCGAGCCAGAGCCATTCGACAACGAAGCGTTCAAAGCCGAAATGCGCGCCGAATAT
GGCGACTGA

Structures

Antitoxin

• AlphaFold DB: H0FXK0