Detailed information of TA system TA077560

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 896632..897230
• Replicon: plasmid psymA
• Accession: NZ_CP021830
• Organism: Sinorhizobium meliloti strain HM006

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q930F0
• Locus tag: CDO22_RS23030
• Protein ID: WP_010967243.1
• Coordinates: 896632..896925 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q930F1
• Locus tag: CDO22_RS23035
• Protein ID: WP_010967242.1
• Coordinates: 896922..897230 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO22_RS23005	892089..893617	+	1529	Protein_849	IS21 family transposase	-
CDO22_RS23010	893630..894367	+	738	WP_088202341.1	IS21-like element helper ATPase IstB	-
CDO22_RS23020	894493..894777	-	285	Protein_851	ParB N-terminal domain-containing protein	-
CDO22_RS23025	895171..896370	+	1200	WP_164820086.1	NAD-dependent formate dehydrogenase	-
CDO22_RS23030	896632..896925	-	294	WP_010967243.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
CDO22_RS23035	896922..897230	-	309	WP_010967242.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
CDO22_RS23040	897456..898349	-	894	WP_192932538.1	LysR family transcriptional regulator	-
CDO22_RS23045	898432..899358	+	927	WP_164832496.1	NmrA/HSCARG family protein	-
CDO22_RS23050	899361..899624	+	264	WP_088202344.1	DUF2798 domain-containing protein	-
CDO22_RS23055	900154..901704	+	1551	WP_088202345.1	calcium-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..1484605	1484605
	-	flank	IS/Tn	-	-	902033..902743	710

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-53)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11278.93 Da
• Isoelectric Point: 6.4734

>T77560 WP_010967243.1 NZ_CP021830:c896925-896632 [Sinorhizobium meliloti]
MRLVWARYALDDRDTIFSYIERENPRAAVHVDEEIVSAVRRLLDFPESGRPGRIAGTRELVIPRTPYIAAYMVMEDRIRI
LRVLHGAQKWPSELDDG

Nucleotide

• Length: 294 bp

>T77560 NZ_CP021830:c896925-896632 [Sinorhizobium meliloti]
ATGAGGCTCGTCTGGGCGCGATATGCGCTTGACGATCGCGACACCATCTTCAGTTACATCGAAAGGGAGAATCCGAGGGC
GGCGGTCCACGTCGACGAGGAGATCGTCAGCGCGGTACGTCGTCTGCTTGATTTTCCGGAAAGCGGCCGCCCTGGCCGGA
TTGCCGGAACTCGAGAGCTTGTTATCCCTCGGACCCCCTACATTGCAGCCTACATGGTAATGGAGGATAGAATTCGAATT
CTGCGTGTCCTGCACGGCGCCCAGAAGTGGCCCAGCGAGCTCGACGACGGGTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11210.58 Da
• Isoelectric Point: 5.6604

>AT77560 WP_010967242.1 NZ_CP021830:c897230-896922 [Sinorhizobium meliloti]
MASNALVQTRIDAEVKERATAVLENMGLTVSDAVRILLTRTANEGALPLELFSHSEAHDAWFRAKVLRALEDTRPDVDDA
DADAHFRERRAAALRKAAAGDR

Nucleotide

• Length: 309 bp

>AT77560 NZ_CP021830:c897230-896922 [Sinorhizobium meliloti]
ATGGCTTCAAATGCACTTGTTCAGACTCGAATCGATGCCGAGGTCAAAGAGCGCGCCACGGCGGTTTTAGAGAACATGGG
TCTGACGGTCTCGGACGCTGTTCGAATCCTGTTAACTCGGACGGCGAATGAGGGCGCCCTGCCGCTGGAACTCTTCAGCC
ACAGCGAGGCGCACGACGCTTGGTTCCGCGCCAAAGTGTTGCGGGCACTCGAGGATACCCGACCCGACGTCGACGATGCC
GACGCCGATGCGCACTTTCGAGAGCGCCGGGCGGCAGCTTTGCGCAAGGCTGCGGCGGGTGATCGATGA

Structures

Toxin

• AlphaFold DB: Q930F0

Antitoxin

• AlphaFold DB: Q930F1