Detailed information of TA system TA077559

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 679338..679873
• Replicon: plasmid psymA
• Accession: NZ_CP021830
• Organism: Sinorhizobium meliloti strain HM006

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CDO22_RS21895
• Protein ID: WP_088202307.1
• Coordinates: 679338..679613 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CDO22_RS21900
• Protein ID: WP_088202308.1
• Coordinates: 679613..679873 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO22_RS21855	674907..675592	-	686	Protein_640	transposase	-
CDO22_RS21860	675702..676043	+	342	Protein_641	ISNCY family transposase	-
CDO22_RS21865	676303..676580	-	278	Protein_642	global cell cycle regulator GcrA	-
CDO22_RS21870	676589..677173	-	585	Protein_643	hypothetical protein	-
CDO22_RS21875	677296..677538	-	243	WP_127662981.1	helix-turn-helix domain-containing protein	-
CDO22_RS21880	677627..678151	+	525	WP_153428689.1	helix-turn-helix domain-containing protein	-
CDO22_RS35935	679038..679112	-	75	Protein_646	YecA family protein	-
CDO22_RS21890	679109..679294	-	186	Protein_647	hypothetical protein	-
CDO22_RS21895	679338..679613	-	276	WP_088202307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDO22_RS21900	679613..679873	-	261	WP_088202308.1	ribbon-helix-helix domain-containing protein	Antitoxin
CDO22_RS21905	680083..680961	-	879	Protein_650	alpha/beta fold hydrolase	-
CDO22_RS21910	680958..681545	-	588	WP_088202275.1	hypothetical protein	-
CDO22_RS21915	681552..682727	-	1176	WP_088202276.1	iron-containing alcohol dehydrogenase	-
CDO22_RS21920	682778..683659	-	882	WP_088202569.1	hypothetical protein	-
CDO22_RS21925	683911..684474	-	564	WP_157718316.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..1484605	1484605
	-	flank	IS/Tn	-	-	675699..675944	245

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10905.54 Da
• Isoelectric Point: 10.5140

>T77559 WP_088202307.1 NZ_CP021830:c679613-679338 [Sinorhizobium meliloti]
MKLEWTRKAVSDLGRLYDFLAPVNRQAAARTVQSLTSAPARLLEQPRIGERLEEFDPREVRRILVGHYEIRFEIRQSTIY
VLRLWHTREER

Nucleotide

• Length: 276 bp

>T77559 NZ_CP021830:c679613-679338 [Sinorhizobium meliloti]
ATGAAGCTCGAGTGGACGCGAAAGGCAGTCTCCGATCTAGGGCGCCTCTATGATTTCCTGGCACCGGTAAATCGACAAGC
CGCTGCTCGCACTGTGCAATCGTTGACAAGCGCTCCAGCACGGCTGCTAGAACAACCGCGCATTGGCGAACGGCTGGAGG
AATTCGACCCACGAGAGGTTCGGCGAATTCTCGTTGGCCATTACGAAATACGGTTTGAGATTCGGCAGTCGACGATCTAC
GTGCTGCGGCTTTGGCATACGCGTGAAGAGCGGTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9734.16 Da
• Isoelectric Point: 4.8308

>AT77559 WP_088202308.1 NZ_CP021830:c679873-679613 [Sinorhizobium meliloti]
METKVLTAHVPLPLAEKVDQIAARLERSRGWIVKQALTAWVDQEEERRRLTLEALADVDASRVIEQHAVQVWADSLDSDK
PLPLPL

Nucleotide

• Length: 261 bp

>AT77559 NZ_CP021830:c679873-679613 [Sinorhizobium meliloti]
ATGGAAACCAAGGTGCTAACCGCGCACGTGCCACTGCCGCTCGCCGAGAAAGTCGATCAGATCGCCGCCCGGCTTGAGCG
CTCGCGCGGGTGGATCGTCAAGCAGGCGCTGACCGCCTGGGTCGATCAGGAAGAGGAGCGCCGACGCTTGACGCTGGAAG
CGCTTGCCGACGTCGATGCTAGCCGCGTGATCGAACAGCATGCCGTTCAGGTCTGGGCCGACAGTCTTGACAGCGACAAG
CCGCTACCCTTGCCGCTGTGA