Detailed information of TA system TA077558

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 360817..361502
• Replicon: plasmid psymA
• Accession: NZ_CP021830
• Organism: Sinorhizobium meliloti strain HM006

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CDO22_RS20020
• Protein ID: WP_088202229.1
• Coordinates: 361083..361502 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F7XET8
• Locus tag: CDO22_RS20015
• Protein ID: WP_013845417.1
• Coordinates: 360817..361086 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO22_RS19985	356453..357322	-	870	WP_014528623.1	aldo/keto reductase family oxidoreductase	-
CDO22_RS19990	357439..358362	+	924	WP_013845420.1	LysR family transcriptional regulator	-
CDO22_RS20005	359342..360217	+	876	WP_013845418.1	LysR family transcriptional regulator	-
CDO22_RS35865	360328..360679	-	352	Protein_332	LysR family transcriptional regulator	-
CDO22_RS20015	360817..361086	+	270	WP_013845417.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CDO22_RS20020	361083..361502	+	420	WP_088202229.1	type II toxin-antitoxin system VapC family toxin	Toxin
CDO22_RS20025	361656..362069	-	414	WP_014528620.1	hypothetical protein	-
CDO22_RS20030	362344..362655	+	312	WP_014528619.1	type II toxin-antitoxin system HigB family toxin	-
CDO22_RS20035	362673..363053	+	381	WP_003526626.1	transcriptional regulator	-
CDO22_RS20040	363323..364696	+	1374	WP_046067457.1	ISNCY family transposase	-
CDO22_RS20045	365056..366482	+	1427	Protein_339	ISNCY family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..1484605	1484605

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (18-55)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14760.06 Da
• Isoelectric Point: 5.1858

>T77558 WP_088202229.1 NZ_CP021830:361083-361502 [Sinorhizobium meliloti]
VSRLYMLDTNIVSELARNPQGAVTKRIAEVGPDAICVSIITAAELRYGCAKKGSSKLLAQIETILGSMRVLALDVPADAE
YGGIRAELEAAGKPIGPNDLFIAAHACMLGAVLVTANSSEFTRVRDLKVENWLDFTPSG

Nucleotide

• Length: 420 bp

>T77558 NZ_CP021830:361083-361502 [Sinorhizobium meliloti]
GTGAGCAGGCTCTACATGCTGGACACCAATATCGTGTCCGAGCTTGCGCGAAACCCACAGGGGGCTGTCACTAAACGCAT
TGCCGAGGTAGGACCGGATGCAATCTGCGTCAGCATCATTACGGCGGCAGAGTTACGCTATGGATGCGCCAAGAAGGGAT
CATCGAAACTACTGGCGCAGATCGAGACCATTCTTGGAAGTATGCGAGTGCTTGCGCTCGATGTGCCTGCTGATGCCGAA
TACGGCGGCATCCGGGCTGAACTGGAGGCCGCTGGTAAGCCCATCGGCCCGAATGACCTGTTCATCGCCGCTCATGCCTG
TATGCTTGGAGCGGTGCTGGTAACGGCCAACAGTAGCGAGTTCACACGCGTTCGTGATCTCAAGGTCGAGAACTGGCTGG
ATTTCACTCCTTCGGGTTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10203.67 Da
• Isoelectric Point: 4.4798

>AT77558 WP_013845417.1 NZ_CP021830:360817-361086 [Sinorhizobium meliloti]
MPELRHNEPTPPREAKLFRNNRSQAVRIPADFELPGDRVMIHRDGDRLIIEPVLRKNLLEVLAGLEPLGSEDEFPDVDET
LLPVKEIDL

Nucleotide

• Length: 270 bp

>AT77558 NZ_CP021830:360817-361086 [Sinorhizobium meliloti]
ATGCCCGAACTCCGACACAACGAACCAACACCGCCAAGGGAAGCTAAGCTCTTCCGAAATAATAGAAGTCAGGCTGTACG
TATCCCCGCTGATTTTGAGCTTCCCGGCGATCGGGTGATGATCCATCGGGACGGAGATCGGCTGATAATAGAGCCGGTGC
TCCGCAAGAACTTGCTGGAGGTGCTGGCTGGTTTGGAGCCGCTCGGGTCTGAAGATGAGTTCCCCGACGTTGACGAAACC
CTGCTGCCGGTGAAGGAAATCGACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A844A3Y5