Detailed information of TA system TA077554

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-RHH
• Location: 1868402..1868976
• Replicon: chromosome
• Accession: NZ_CP021829
• Organism: Sinorhizobium meliloti strain HM006

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q92QN4
• Locus tag: CDO22_RS09035
• Protein ID: WP_010969154.1
• Coordinates: 1868402..1868767 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CDO22_RS09040
• Protein ID: WP_017276849.1
• Coordinates: 1868761..1868976 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO22_RS09010	1863542..1864984	+	1443	WP_010969150.1	NADH-quinone oxidoreductase subunit NuoN	-
CDO22_RS09015	1864981..1865748	+	768	WP_003531861.1	biotin--[acetyl-CoA-carboxylase] ligase	-
CDO22_RS09020	1865776..1867443	+	1668	WP_010969151.1	ribonuclease J	-
CDO22_RS09025	1867502..1867906	+	405	WP_010969152.1	methylmalonyl-CoA epimerase	-
CDO22_RS09030	1868046..1868315	+	270	WP_014526683.1	DUF1467 family protein	-
CDO22_RS09035	1868402..1868767	-	366	WP_010969154.1	type II toxin-antitoxin system VapC family toxin	Toxin
CDO22_RS09040	1868761..1868976	-	216	WP_017276849.1	ribbon-helix-helix protein, CopG family	Antitoxin
CDO22_RS09045	1869044..1869271	-	228	WP_017264047.1	hypothetical protein	-
CDO22_RS09050	1869687..1871015	+	1329	WP_003531873.1	proline--tRNA ligase	-
CDO22_RS09055	1871036..1872346	+	1311	WP_014529722.1	lipoprotein-releasing ABC transporter permease subunit	-
CDO22_RS09060	1872364..1873050	+	687	WP_010969157.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-106)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-40)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13592.57 Da
• Isoelectric Point: 5.1787

>T77554 WP_010969154.1 NZ_CP021829:c1868767-1868402 [Sinorhizobium meliloti]
MVGALFDTNILIDHLNAVPQAHKELDRFENRAISIITWMEVMVGADAELVEPTRRFLDGFETIALNDEIANRAVTLRRAH
RIKLPDAVIWATAQTAGRLLVTRNTKDFPADDPGIREPYAV

Nucleotide

• Length: 366 bp

>T77554 NZ_CP021829:c1868767-1868402 [Sinorhizobium meliloti]
ATGGTAGGCGCACTGTTCGATACCAATATCCTTATCGATCATCTGAATGCGGTGCCTCAGGCGCACAAGGAACTTGACCG
TTTCGAAAACCGGGCCATCAGCATCATCACCTGGATGGAGGTGATGGTTGGAGCGGACGCCGAACTGGTTGAGCCGACCC
GGCGCTTCCTCGACGGTTTCGAAACGATCGCCTTGAACGATGAGATCGCCAACCGTGCGGTGACATTGCGCAGGGCGCAC
CGCATCAAGCTACCCGACGCGGTGATCTGGGCAACGGCGCAGACTGCCGGTCGTCTGCTCGTGACGCGCAATACCAAGGA
CTTTCCAGCCGACGACCCGGGGATACGCGAACCCTACGCCGTGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8411.53 Da
• Isoelectric Point: 8.6052

>AT77554 WP_017276849.1 NZ_CP021829:c1868976-1868761 [Sinorhizobium meliloti]
MRALIDMNDTQVEALDTLAKRVRRSRAALIREAIDDYLNRHHREQIEDGFGLWSKRKVDGLAYQEKVRGEW

Nucleotide

• Length: 216 bp

>AT77554 NZ_CP021829:c1868976-1868761 [Sinorhizobium meliloti]
ATGCGAGCCTTGATCGATATGAACGATACCCAGGTCGAAGCCCTAGATACCCTCGCCAAGCGGGTGCGCCGGTCCCGTGC
GGCTCTGATCCGCGAAGCGATCGACGACTATCTCAACCGTCATCATCGCGAGCAGATCGAAGATGGCTTCGGTTTGTGGA
GCAAGCGCAAAGTTGACGGTCTGGCTTATCAGGAGAAGGTGCGCGGCGAATGGTAG

Structures

Toxin

• AlphaFold DB: Q92QN4