Detailed information of TA system TA077551

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 1115081..1115658
• Replicon: chromosome
• Accession: NZ_CP021829
• Organism: Sinorhizobium meliloti strain HM006

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: Q92S20
• Locus tag: CDO22_RS05205
• Protein ID: WP_010968707.1
• Coordinates: 1115081..1115344 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q92KN5
• Locus tag: CDO22_RS05210
• Protein ID: WP_010968708.1
• Coordinates: 1115341..1115658 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO22_RS05165	1110410..1110850	+	441	WP_010968702.1	DUF2948 family protein	-
CDO22_RS05170	1110915..1112219	+	1305	WP_010968703.1	histidinol dehydrogenase	-
CDO22_RS05175	1112216..1112692	+	477	WP_010968704.1	UPF0262 family protein	-
CDO22_RS05180	1112706..1113185	+	480	WP_010968705.1	low molecular weight phosphatase family protein	-
CDO22_RS05185	1113343..1113561	+	219	WP_004435948.1	translation initiation factor IF-1	-
CDO22_RS05190	1113659..1114279	+	621	WP_004435951.1	Maf-like protein	-
CDO22_RS05195	1114284..1114496	+	213	WP_010968706.1	DNA gyrase inhibitor YacG	-
CDO22_RS05205	1115081..1115344	+	264	WP_010968707.1	BrnT family toxin	Toxin
CDO22_RS05210	1115341..1115658	+	318	WP_010968708.1	BrnA antitoxin family protein	Antitoxin
CDO22_RS05215	1115861..1116325	-	465	WP_013844019.1	type II toxin-antitoxin system HicB family antitoxin	-
CDO22_RS05220	1116322..1116510	-	189	WP_013844020.1	hypothetical protein	-
CDO22_RS05225	1116830..1117930	+	1101	WP_010968710.1	hypothetical protein	-
CDO22_RS05230	1117917..1118999	+	1083	WP_010968711.1	nucleotidyltransferase family protein	-
CDO22_RS05235	1119202..1119513	+	312	WP_004435964.1	hypothetical protein	-
CDO22_RS05240	1119793..1120182	-	390	WP_013844021.1	DUF930 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-82)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (26-103)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 9821.16 Da
• Isoelectric Point: 10.3413

>T77551 WP_010968707.1 NZ_CP021829:1115081-1115344 [Sinorhizobium meliloti]
MRIVWGEPKRRANLEKHGFDFADVSDLDWASALIEDSKPDTSGRKRLKAIGYFRDGTAAVIFATLGTEAISIISFRPASD
RERRRLP

Nucleotide

• Length: 264 bp

>T77551 NZ_CP021829:1115081-1115344 [Sinorhizobium meliloti]
ATGCGAATTGTGTGGGGCGAACCCAAACGGCGAGCCAACCTTGAGAAGCATGGTTTCGATTTTGCCGATGTAAGCGACCT
CGATTGGGCGAGTGCCCTCATAGAGGACAGCAAGCCAGATACTTCTGGCAGAAAGCGCCTGAAGGCTATCGGTTATTTTC
GTGACGGGACGGCAGCGGTCATTTTCGCGACACTCGGAACGGAAGCTATATCGATCATCAGCTTTCGTCCTGCAAGCGAC
AGAGAAAGAAGGAGGCTTCCATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12197.83 Da
• Isoelectric Point: 10.0883

>AT77551 WP_010968708.1 NZ_CP021829:1115341-1115658 [Sinorhizobium meliloti]
MTTRTRKSKTFEPGHGYTKEEWDAVDFPELTDEELTNMRPARDVLPPEFFKAMEEHRRSRGRPSLEHPKKQVTLRLDEDV
IAKFRAGGKGWQSRMNEALRKAAGI

Nucleotide

• Length: 318 bp

>AT77551 NZ_CP021829:1115341-1115658 [Sinorhizobium meliloti]
ATGACTACCAGGACCCGAAAATCGAAAACGTTCGAGCCAGGTCACGGCTATACAAAAGAGGAATGGGATGCAGTCGATTT
TCCCGAGTTGACCGATGAAGAGCTTACCAACATGCGTCCCGCAAGAGATGTGCTGCCGCCTGAGTTTTTCAAAGCTATGG
AGGAGCATCGCAGATCGCGTGGGCGCCCCTCGCTGGAGCATCCGAAAAAGCAAGTCACCCTCCGGTTGGACGAAGACGTG
ATCGCTAAGTTTCGAGCTGGTGGCAAAGGCTGGCAGAGCCGGATGAATGAGGCGCTGCGAAAAGCTGCAGGTATATAG

Structures

Toxin

• AlphaFold DB: Q92S20

Antitoxin

• AlphaFold DB: Q92KN5