Detailed information of TA system TA077546

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 734758..735443
• Replicon: plasmid psymA
• Accession: NZ_CP021827
• Organism: Sinorhizobium meliloti strain KH35c

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CDO23_RS22680
• Protein ID: WP_017263896.1
• Coordinates: 734758..735177 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F7XET8
• Locus tag: CDO23_RS22685
• Protein ID: WP_013845417.1
• Coordinates: 735174..735443 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO23_RS22645	729772..731025	+	1254	WP_088218135.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
CDO23_RS22650	731080..731439	+	360	WP_088218136.1	hypothetical protein	-
CDO23_RS22655	731484..731757	-	274	Protein_677	helix-turn-helix domain-containing protein	-
CDO23_RS22660	731945..732946	-	1002	WP_088218137.1	IS30 family transposase	-
CDO23_RS22665	733206..733586	-	381	WP_003526626.1	transcriptional regulator	-
CDO23_RS22670	733604..733915	-	312	WP_014528619.1	type II toxin-antitoxin system HigB family toxin	-
CDO23_RS22675	734190..734603	+	414	WP_014528620.1	hypothetical protein	-
CDO23_RS22680	734758..735177	-	420	WP_017263896.1	type II toxin-antitoxin system VapC family toxin	Toxin
CDO23_RS22685	735174..735443	-	270	WP_013845417.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CDO23_RS34310	735581..735932	+	352	Protein_684	LysR family transcriptional regulator	-
CDO23_RS22695	736046..736918	-	873	Protein_685	LysR family transcriptional regulator	-
CDO23_RS22710	737898..738821	-	924	WP_088218138.1	LysR family transcriptional regulator	-
CDO23_RS22715	738938..739807	+	870	WP_014528623.1	aldo/keto reductase family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..1279723	1279723
	-	flank	IS/Tn	-	-	731945..732946	1001

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (18-55)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.00 Da
• Isoelectric Point: 4.8722

>T77546 WP_017263896.1 NZ_CP021827:c735177-734758 [Sinorhizobium meliloti]
VSRLYMLDTNIVSELARNPQGAVTKRIAEVGPDAICVSIITAAELRYGCAKKGSSKLLAQIETILGSMQVLALDVPADAE
YGGIRAELEAAGKPIGPNDLFIAAHACMLGAVLVTANSSEFTRVRDLKVENWLDFTPSG

Nucleotide

• Length: 420 bp

>T77546 NZ_CP021827:c735177-734758 [Sinorhizobium meliloti]
GTGAGCAGGCTCTACATGCTGGACACCAATATCGTGTCCGAGCTTGCGCGAAACCCACAGGGGGCTGTCACTAAACGCAT
TGCCGAGGTAGGACCGGATGCAATCTGCGTCAGCATCATTACGGCGGCAGAGTTACGCTATGGATGCGCCAAGAAGGGAT
CATCGAAACTACTGGCGCAGATCGAGACCATTCTTGGAAGTATGCAAGTGCTTGCGCTCGATGTGCCTGCTGATGCCGAA
TACGGCGGCATCCGGGCTGAACTGGAGGCCGCTGGTAAGCCCATCGGCCCGAATGACCTGTTCATCGCCGCTCATGCCTG
TATGCTTGGAGCGGTGCTGGTAACGGCCAACAGTAGCGAGTTCACACGCGTTCGTGATCTCAAGGTCGAGAACTGGCTGG
ATTTCACTCCTTCGGGTTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10203.67 Da
• Isoelectric Point: 4.4798

>AT77546 WP_013845417.1 NZ_CP021827:c735443-735174 [Sinorhizobium meliloti]
MPELRHNEPTPPREAKLFRNNRSQAVRIPADFELPGDRVMIHRDGDRLIIEPVLRKNLLEVLAGLEPLGSEDEFPDVDET
LLPVKEIDL

Nucleotide

• Length: 270 bp

>AT77546 NZ_CP021827:c735443-735174 [Sinorhizobium meliloti]
ATGCCCGAACTCCGACACAACGAACCAACACCGCCAAGGGAAGCTAAGCTCTTCCGAAATAATAGAAGTCAGGCTGTACG
TATCCCCGCTGATTTTGAGCTTCCCGGCGATCGGGTGATGATCCATCGGGACGGAGATCGGCTGATAATAGAGCCGGTGC
TCCGCAAGAACTTGCTGGAGGTGCTGGCTGGTTTGGAGCCGCTCGGGTCTGAAGATGAGTTCCCCGACGTTGACGAAACC
CTGCTGCCGGTGAAGGAAATCGACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A844A3Y5