77543

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 3092895..3093460 Replicon chromosome
Accession NZ_CP021825
Organism Sinorhizobium meliloti strain KH35c

Toxin (Protein)


Gene name brnT Uniprot ID H0G3P4
Locus tag CDO23_RS15340 Protein ID WP_003531565.1
Coordinates 3092895..3093140 (+) Length 82 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID H0G3P5
Locus tag CDO23_RS15345 Protein ID WP_003531566.1
Coordinates 3093137..3093460 (+) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDO23_RS15315 3088127..3089272 - 1146 WP_028009695.1 alpha-hydroxy-acid oxidizing protein -
CDO23_RS15320 3089360..3089977 - 618 WP_028009694.1 hypothetical protein -
CDO23_RS15325 3090186..3090674 - 489 WP_028009693.1 RidA family protein -
CDO23_RS15330 3090867..3091562 + 696 WP_028009692.1 TetR/AcrR family transcriptional regulator C-terminal domain-containing protein -
CDO23_RS15335 3091747..3092460 - 714 WP_013843872.1 replicative DNA helicase -
CDO23_RS15340 3092895..3093140 + 246 WP_003531565.1 BrnT family toxin Toxin
CDO23_RS15345 3093137..3093460 + 324 WP_003531566.1 BrnA antitoxin family protein Antitoxin
CDO23_RS15355 3093757..3095277 - 1521 WP_027989491.1 HAMP domain-containing histidine kinase -
CDO23_RS15365 3095352..3096773 - 1422 WP_028009691.1 NAD(P)/FAD-dependent oxidoreductase -
CDO23_RS15370 3096796..3097596 - 801 WP_028009690.1 TVP38/TMEM64 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-77)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-101)


Sequences

Toxin        


Download         Length: 82 a.a.        Molecular weight: 8990.48 Da        Isoelectric Point: 9.7323

>T77543 WP_003531565.1 NZ_CP021825:3092895-3093140 [Sinorhizobium meliloti]
MKVTYDETKRQINIGKHGYDIAELDMEFFASSVVVPAKEGRFMAIGVLRNGVIAVVFARLGSEGISIISMRDASRKERSL
L

Download         Length: 246 bp

>T77543 NZ_CP021825:3092895-3093140 [Sinorhizobium meliloti]
ATGAAGGTCACCTATGACGAAACAAAGCGCCAGATCAACATAGGAAAGCACGGCTATGATATCGCCGAACTCGACATGGA
GTTCTTCGCTTCGTCTGTGGTGGTCCCAGCCAAGGAAGGCCGCTTCATGGCTATCGGCGTTTTGCGCAATGGCGTTATCG
CCGTAGTCTTCGCCCGGCTTGGCTCGGAAGGCATTTCGATTATCTCCATGCGTGACGCCAGCAGGAAGGAAAGGAGCTTA
TTATGA

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 11933.51 Da        Isoelectric Point: 10.2830

>AT77543 WP_003531566.1 NZ_CP021825:3093137-3093460 [Sinorhizobium meliloti]
MTSLSRRIRHISDKEEAAIQRKIASDPDAPEATDEQLAKAKPFAEAFPDLAESIKRARGRPAVENPRQQISIRLDPDVIE
HYKATGKGWQSRMNDDLRKAAGLKKAG

Download         Length: 324 bp

>AT77543 NZ_CP021825:3093137-3093460 [Sinorhizobium meliloti]
ATGACCAGCCTATCCCGCCGCATCCGCCATATCTCCGACAAGGAGGAGGCAGCTATCCAACGCAAGATAGCTAGTGACCC
AGACGCTCCTGAGGCCACCGACGAACAACTAGCCAAAGCTAAGCCATTCGCCGAAGCCTTCCCAGACCTGGCCGAGAGCA
TCAAGCGCGCCAGGGGCCGTCCAGCCGTTGAGAACCCTAGGCAGCAGATATCCATTCGCCTCGATCCGGATGTCATCGAG
CACTACAAGGCAACCGGCAAAGGCTGGCAGAGCCGGATGAATGACGACCTTAGAAAGGCTGCCGGATTGAAGAAGGCAGG
TTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB H0G3P4

Antitoxin

Source ID Structure
AlphaFold DB H0G3P5

References