Detailed information of TA system TA077541

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 1384724..1385354
• Replicon: chromosome
• Accession: NZ_CP021825
• Organism: Sinorhizobium meliloti strain KH35c

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: CDO23_RS06790
• Protein ID: WP_028011798.1
• Coordinates: 1385166..1385354 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: H0G1A2
• Locus tag: CDO23_RS06785
• Protein ID: WP_003530007.1
• Coordinates: 1384724..1385161 (-)
• Length: 146 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO23_RS06760	1381209..1381598	+	390	WP_088217725.1	helix-turn-helix domain-containing protein	-
CDO23_RS06765	1381749..1382117	-	369	WP_088217726.1	hypothetical protein	-
CDO23_RS34175	1382110..1382280	-	171	WP_088217860.1	hypothetical protein	-
CDO23_RS06775	1382373..1383347	+	975	WP_088217727.1	non-homologous end-joining DNA ligase	-
CDO23_RS06780	1383363..1384274	-	912	WP_088217728.1	hypothetical protein	-
CDO23_RS06785	1384724..1385161	-	438	WP_003530007.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CDO23_RS06790	1385166..1385354	-	189	WP_028011798.1	type II toxin-antitoxin system HicA family toxin	Toxin
CDO23_RS06795	1385531..1386301	+	771	WP_028011797.1	LpsS	-
CDO23_RS06800	1386752..1387723	+	972	WP_028011858.1	glycosyltransferase family 2 protein	-
CDO23_RS06805	1387748..1389058	-	1311	WP_028011859.1	HlyD family type I secretion periplasmic adaptor subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1331494..1416863	85369

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 6955.13 Da
• Isoelectric Point: 11.0889

>T77541 WP_028011798.1 NZ_CP021825:c1385354-1385166 [Sinorhizobium meliloti]
VKSGDIIAALQKDGWYEVATKGSHVQFKHPKKHGRVTVLHPKRDLPIGTLRSIEKQSGLKLR

Nucleotide

• Length: 189 bp

>T77541 NZ_CP021825:c1385354-1385166 [Sinorhizobium meliloti]
GTGAAGAGCGGCGACATTATTGCAGCATTGCAGAAGGACGGATGGTACGAGGTTGCGACCAAGGGCAGCCACGTTCAATT
CAAACATCCGAAAAAGCACGGCCGGGTTACCGTTCTTCACCCGAAGAGGGATCTACCAATCGGTACCCTCAGGAGCATTG
AAAAGCAGTCCGGTTTGAAATTGAGGTAA

Antitoxin

Protein

• Length: 146 a.a.
• Molecular weight: 15506.32 Da
• Isoelectric Point: 4.3251

>AT77541 WP_003530007.1 NZ_CP021825:c1385161-1384724 [Sinorhizobium meliloti]
MRNYIGLIHKDAESDYGVSFPDFSGVVTAGADLDDARAMAEEALALHIEGLVEDGEAIPEPSSLEVVMSDVENKDCVAIL
VAVKTEAKRAIRVNVTLPEGVLKQIDAFAEAHGLTRSGFLARAATHEIERANDGHDAYAESRLSA

Nucleotide

• Length: 438 bp

>AT77541 NZ_CP021825:c1385161-1384724 [Sinorhizobium meliloti]
ATGCGCAACTATATCGGATTGATCCACAAGGACGCCGAGAGCGATTACGGCGTTTCCTTTCCTGATTTTTCCGGCGTCGT
GACCGCCGGTGCCGACCTCGACGACGCGCGCGCTATGGCAGAGGAAGCCTTGGCCTTGCATATTGAAGGGCTGGTAGAGG
ACGGCGAGGCCATCCCGGAGCCCTCCTCCCTTGAAGTTGTCATGTCCGACGTCGAGAACAAGGATTGCGTCGCTATTCTC
GTCGCGGTGAAAACCGAGGCAAAAAGAGCCATTCGAGTGAACGTTACGCTTCCCGAAGGTGTCCTGAAGCAGATAGATGC
CTTCGCCGAAGCCCACGGCCTTACGCGGTCTGGATTTCTTGCTCGTGCTGCAACGCACGAGATCGAACGGGCAAATGACG
GCCACGACGCTTATGCGGAATCCCGCTTGTCGGCTTAG

Structures

Antitoxin

• AlphaFold DB: H0G1A2