Detailed information of TA system TA077540

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 923458..924131
• Replicon: chromosome
• Accession: NZ_CP021825
• Organism: Sinorhizobium meliloti strain KH35c

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CDO23_RS04415
• Protein ID: WP_028010497.1
• Coordinates: 923458..923862 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CDO23_RS04420
• Protein ID: WP_028010496.1
• Coordinates: 923859..924131 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO23_RS04370	918670..918810	-	141	WP_027990619.1	hemin uptake protein HemP	-
CDO23_RS04375	919040..919474	+	435	WP_027990618.1	hypothetical protein	-
CDO23_RS04380	919515..920441	+	927	WP_028011594.1	energy transducer TonB	-
CDO23_RS04385	920647..921060	+	414	WP_003536357.1	DUF2325 domain-containing protein	-
CDO23_RS04390	921113..921379	+	267	WP_028011595.1	hypothetical protein	-
CDO23_RS04395	921662..921976	+	315	WP_028010499.1	antibiotic biosynthesis monooxygenase	-
CDO23_RS04400	922193..922435	-	243	WP_050592581.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
CDO23_RS04405	922558..922932	-	375	WP_027990613.1	DUF423 domain-containing protein	-
CDO23_RS04410	922938..923330	-	393	WP_028010498.1	globin	-
CDO23_RS04415	923458..923862	-	405	WP_028010497.1	type II toxin-antitoxin system VapC family toxin	Toxin
CDO23_RS04420	923859..924131	-	273	WP_028010496.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CDO23_RS04425	924268..924828	+	561	WP_028010495.1	TetR/AcrR family transcriptional regulator	-
CDO23_RS04430	924876..925217	+	342	WP_003536339.1	EamA family transporter	-
CDO23_RS04435	925397..926452	+	1056	WP_028010494.1	dipeptidase	-
CDO23_RS04440	926500..928140	+	1641	WP_003536336.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (16-54)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14732.97 Da
• Isoelectric Point: 4.9000

>T77540 WP_028010497.1 NZ_CP021825:c923862-923458 [Sinorhizobium meliloti]
MNGYLLDTNIISDVIHNPFGPAAQRIERIGPKEIYTSIVVASELRYGCAIKGSAKLLAKVESLLEIVPVLPLDIPADTRY
GSIRAELESLGQTIGSNDLLIAAHAYALDLTLVTDNIREFSRVRGLSLENWLER

Nucleotide

• Length: 405 bp

>T77540 NZ_CP021825:c923862-923458 [Sinorhizobium meliloti]
TTGAACGGGTATCTTCTCGATACGAACATCATCAGCGACGTCATTCACAATCCGTTCGGACCGGCTGCGCAACGCATCGA
ACGGATCGGCCCGAAGGAGATTTACACCAGCATCGTCGTCGCGTCCGAGTTGCGGTATGGTTGCGCAATAAAGGGATCGG
CGAAGTTGCTCGCCAAGGTCGAAAGCTTGCTGGAAATCGTCCCGGTGCTGCCATTGGATATTCCGGCCGACACCCGATAT
GGCAGCATTCGTGCGGAATTGGAGTCTCTGGGGCAGACCATCGGCTCCAACGATCTGTTGATTGCCGCGCATGCATATGC
GCTGGACCTGACGCTGGTGACGGACAATATCCGTGAGTTCAGCCGCGTTCGTGGCCTTAGTCTGGAAAACTGGCTTGAGC
GATAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10305.97 Da
• Isoelectric Point: 5.2300

>AT77540 WP_028010496.1 NZ_CP021825:c924131-923859 [Sinorhizobium meliloti]
MPVPLPSSRPKEVKLFRNNRSQAVRIPAEFELPGDRVLIRREGTRLIIEPIARPADIVELLAEWKKEAPLGPEDQFPDVE
DIPARPEKIF

Nucleotide

• Length: 273 bp

>AT77540 NZ_CP021825:c924131-923859 [Sinorhizobium meliloti]
ATGCCCGTCCCGTTGCCGTCATCGCGACCTAAAGAAGTAAAGCTGTTCCGCAATAACCGCAGCCAGGCAGTCCGTATTCC
TGCGGAGTTCGAGTTGCCTGGAGACCGTGTGTTGATCCGTCGCGAAGGAACTAGACTCATTATCGAACCGATAGCCAGGC
CAGCCGATATCGTGGAGCTTCTCGCGGAGTGGAAGAAGGAAGCTCCCCTCGGACCTGAAGACCAGTTCCCAGATGTCGAG
GATATACCCGCCCGGCCGGAGAAAATCTTTTGA