Detailed information of TA system TA077539

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacT-ataR/DUF1778(antitoxin)
• Location: 681457..682223
• Replicon: chromosome
• Accession: NZ_CP021825
• Organism: Sinorhizobium meliloti strain KH35c

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: H0G6D3
• Locus tag: CDO23_RS03225
• Protein ID: WP_003533488.1
• Coordinates: 681457..681939 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: H0G6D4
• Locus tag: CDO23_RS03230
• Protein ID: WP_003533490.1
• Coordinates: 681939..682223 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO23_RS03195	677198..678073	+	876	WP_010970093.1	YihY/virulence factor BrkB family protein	-
CDO23_RS03200	678092..678988	-	897	WP_028010990.1	tRNA glutamyl-Q(34) synthetase GluQRS	-
CDO23_RS03205	679141..679788	+	648	WP_027993538.1	DNA-3-methyladenine glycosylase	-
CDO23_RS03210	679894..680451	+	558	WP_003533486.1	HNH endonuclease	-
CDO23_RS03215	680483..680992	-	510	WP_028010991.1	disulfide bond formation protein B	-
CDO23_RS03225	681457..681939	-	483	WP_003533488.1	GNAT family N-acetyltransferase	Toxin
CDO23_RS03230	681939..682223	-	285	WP_003533490.1	DUF1778 domain-containing protein	Antitoxin
CDO23_RS03235	682428..684074	+	1647	WP_028010992.1	AMP-binding protein	-
CDO23_RS03240	684086..685357	-	1272	WP_010970087.1	GNAT family N-acetyltransferase	-
CDO23_RS03245	685361..686749	-	1389	WP_028010993.1	lipopolysaccharide biosynthesis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-92)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17463.88 Da
• Isoelectric Point: 7.3827

>T77539 WP_003533488.1 NZ_CP021825:c681939-681457 [Sinorhizobium meliloti]
MLSAPTPLGEAHDFDLFQSGNDTLDDWLRRRAHANQASGASRTYVIAEEWRVVGYYCLASGALDLADAPSSVRRNMPDPI
PMAVLGRLAIDRDWQGKGLGAALLQDAVLRSSQAADIMGIRGLLVHAISGEAKAFYEHYGFQCSPNHPMTLVLSLKGKRR

Nucleotide

• Length: 483 bp

>T77539 NZ_CP021825:c681939-681457 [Sinorhizobium meliloti]
ATGCTGTCGGCGCCGACGCCTCTTGGTGAAGCCCACGACTTCGATCTTTTCCAGAGCGGCAACGACACGCTCGATGACTG
GCTGCGCCGCCGTGCGCACGCTAATCAGGCCAGTGGCGCGTCGCGCACCTATGTTATCGCTGAAGAATGGCGCGTGGTCG
GCTATTACTGCCTTGCATCCGGTGCCCTCGATCTGGCCGACGCGCCGAGCTCTGTCCGGCGCAACATGCCGGATCCCATC
CCGATGGCGGTGCTCGGTCGCCTTGCTATCGACCGCGACTGGCAGGGCAAAGGGTTGGGCGCGGCGCTATTGCAAGATGC
CGTCCTGCGGTCCTCGCAGGCGGCCGACATCATGGGAATACGGGGCTTGCTCGTGCATGCCATCTCGGGCGAGGCGAAAG
CCTTTTACGAACATTACGGCTTCCAGTGCTCACCCAACCATCCCATGACGCTGGTGTTGTCGCTCAAGGGCAAGCGCCGT
TAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10771.12 Da
• Isoelectric Point: 7.3175

>AT77539 WP_003533490.1 NZ_CP021825:c682223-681939 [Sinorhizobium meliloti]
MATKSSTDTHAKPVNLRIREDIRKVIDRAASIRGKTRSDFMIDAAYRAAEDTLLDQTLVRVDEHSYRHYLDILDQPPSGD
GFERLMNAPKPWQS

Nucleotide

• Length: 285 bp

>AT77539 NZ_CP021825:c682223-681939 [Sinorhizobium meliloti]
ATGGCCACGAAATCGTCGACTGACACCCACGCGAAGCCCGTCAACCTGCGAATCCGAGAGGATATACGCAAGGTGATCGA
TCGCGCCGCGAGCATTCGCGGCAAGACCCGCTCCGATTTTATGATCGACGCAGCCTACCGCGCGGCCGAAGACACGCTGC
TCGATCAGACGCTGGTGCGGGTCGATGAGCACAGCTATCGGCACTACCTAGACATTCTTGACCAGCCGCCAAGTGGCGAC
GGCTTCGAACGCCTGATGAATGCGCCGAAACCCTGGCAGTCCTGA

Structures

Toxin

• AlphaFold DB: H0G6D3

Antitoxin

• AlphaFold DB: H0G6D4