Detailed information of TA system TA077537

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 845032..845717
• Replicon: plasmid psymA
• Accession: NZ_CP021824
• Organism: Sinorhizobium meliloti strain KH46

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CDO24_RS30945
• Protein ID: WP_017263896.1
• Coordinates: 845298..845717 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F7XET8
• Locus tag: CDO24_RS30940
• Protein ID: WP_013845417.1
• Coordinates: 845032..845301 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO24_RS30910	840668..841537	-	870	WP_014528623.1	aldo/keto reductase family oxidoreductase	-
CDO24_RS30915	841654..842577	+	924	WP_014528622.1	LysR family transcriptional regulator	-
CDO24_RS30930	843557..844432	+	876	WP_013845418.1	LysR family transcriptional regulator	-
CDO24_RS30940	845032..845301	+	270	WP_013845417.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CDO24_RS30945	845298..845717	+	420	WP_017263896.1	type II toxin-antitoxin system VapC family toxin	Toxin
CDO24_RS30950	845871..846284	-	414	WP_014528620.1	hypothetical protein	-
CDO24_RS30955	846559..846870	+	312	WP_014528619.1	type II toxin-antitoxin system HigB family toxin	-
CDO24_RS30960	846888..847268	+	381	WP_003526626.1	transcriptional regulator	-
CDO24_RS30965	847528..848529	+	1002	WP_013851102.1	IS30 family transposase	-
CDO24_RS30970	848655..850028	+	1374	WP_014531577.1	ISNCY family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..1597380	1597380

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (18-55)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.00 Da
• Isoelectric Point: 4.8722

>T77537 WP_017263896.1 NZ_CP021824:845298-845717 [Sinorhizobium meliloti]
VSRLYMLDTNIVSELARNPQGAVTKRIAEVGPDAICVSIITAAELRYGCAKKGSSKLLAQIETILGSMQVLALDVPADAE
YGGIRAELEAAGKPIGPNDLFIAAHACMLGAVLVTANSSEFTRVRDLKVENWLDFTPSG

Nucleotide

• Length: 420 bp

>T77537 NZ_CP021824:845298-845717 [Sinorhizobium meliloti]
GTGAGCAGGCTCTACATGCTGGACACCAATATCGTGTCCGAGCTTGCGCGAAACCCACAGGGGGCTGTCACTAAACGCAT
TGCCGAGGTAGGACCGGATGCAATCTGCGTCAGCATCATTACGGCGGCAGAGTTACGCTATGGATGCGCCAAGAAGGGAT
CATCGAAACTACTGGCGCAGATCGAGACCATTCTTGGAAGTATGCAAGTGCTTGCGCTCGATGTGCCTGCTGATGCCGAA
TACGGCGGCATCCGGGCTGAACTGGAGGCCGCTGGTAAGCCCATCGGCCCGAATGACCTGTTCATCGCCGCTCATGCCTG
TATGCTTGGAGCGGTGCTGGTAACGGCCAACAGTAGCGAGTTCACACGCGTTCGTGATCTCAAGGTCGAGAACTGGCTGG
ATTTCACTCCTTCGGGTTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10203.67 Da
• Isoelectric Point: 4.4798

>AT77537 WP_013845417.1 NZ_CP021824:845032-845301 [Sinorhizobium meliloti]
MPELRHNEPTPPREAKLFRNNRSQAVRIPADFELPGDRVMIHRDGDRLIIEPVLRKNLLEVLAGLEPLGSEDEFPDVDET
LLPVKEIDL

Nucleotide

• Length: 270 bp

>AT77537 NZ_CP021824:845032-845301 [Sinorhizobium meliloti]
ATGCCCGAACTCCGACACAACGAACCAACACCGCCAAGGGAAGCTAAGCTCTTCCGAAATAATAGAAGTCAGGCTGTACG
TATCCCCGCTGATTTTGAGCTTCCCGGCGATCGGGTGATGATCCATCGGGACGGAGATCGGCTGATAATAGAGCCGGTGC
TCCGCAAGAACTTGCTGGAGGTGCTGGCTGGTTTGGAGCCGCTCGGGTCTGAAGATGAGTTCCCCGACGTTGACGAAACC
CTGCTGCCGGTGAAGGAAATCGACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A844A3Y5