Detailed information of TA system TA077535

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 342039..342646
• Replicon: plasmid psymA
• Accession: NZ_CP021824
• Organism: Sinorhizobium meliloti strain KH46

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: CDO24_RS28345
• Protein ID: WP_017264738.1
• Coordinates: 342392..342646 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CDO24_RS28340
• Protein ID: WP_040120685.1
• Coordinates: 342039..342395 (-)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO24_RS28320	337547..339040	+	1494	WP_010968049.1	sugar ABC transporter ATP-binding protein	-
CDO24_RS28325	339057..340028	+	972	WP_040120683.1	ABC transporter permease	-
CDO24_RS28330	340031..340981	+	951	WP_010968047.1	ABC transporter permease	-
CDO24_RS28335	341011..341667	+	657	WP_010968046.1	SGNH/GDSL hydrolase family protein	-
CDO24_RS28340	342039..342395	-	357	WP_040120685.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CDO24_RS28345	342392..342646	-	255	WP_017264738.1	type II toxin-antitoxin system HicA family toxin	Toxin
CDO24_RS28350	342988..343875	-	888	WP_040120686.1	LysR family transcriptional regulator	-
CDO24_RS28355	343964..345004	+	1041	WP_040120687.1	NAD(P)H-binding protein	-
CDO24_RS35970	345128..345286	+	159	WP_017276730.1	hypothetical protein	-
CDO24_RS35525	345372..345527	+	156	WP_153320124.1	hypothetical protein	-
CDO24_RS28360	345578..346450	-	873	WP_010968043.1	LysR family transcriptional regulator	-
CDO24_RS28365	346541..347245	+	705	WP_088200055.1	haloacid dehalogenase type II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..1597380	1597380

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9294.62 Da
• Isoelectric Point: 9.8124

>T77535 WP_017264738.1 NZ_CP021824:c342646-342392 [Sinorhizobium meliloti]
MNSKQRKTLAAVFKDPASGTIEWAAIENLLVAAGADVIEGNGSRVKFEKDGIIASFHRPHPDKEAKRYQVRDAREFLMQI
GVTP

Nucleotide

• Length: 255 bp

>T77535 NZ_CP021824:c342646-342392 [Sinorhizobium meliloti]
ATGAACAGCAAGCAGAGAAAGACCCTCGCGGCCGTATTCAAGGACCCGGCCTCGGGAACGATTGAATGGGCCGCTATAGA
GAATCTGCTTGTGGCTGCCGGAGCGGACGTCATTGAGGGGAACGGTTCCCGCGTTAAGTTTGAAAAAGACGGCATCATTG
CGAGCTTTCATCGACCTCATCCGGACAAGGAAGCAAAGCGTTATCAGGTGCGCGATGCTCGCGAGTTCCTGATGCAGATT
GGAGTAACGCCATGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 13178.82 Da
• Isoelectric Point: 5.2288

>AT77535 WP_040120685.1 NZ_CP021824:c342395-342039 [Sinorhizobium meliloti]
MNTMTYNGYHARIEFDAEDEVFFGKIAGISDVIGFHGDSVAELKKAFHEAVDDYLETCRKIGKEPQRPYSGKMMFRVAPE
VHRRAALAAELSGKSLNQWAEEVLEEAAGHFAEARLSA

Nucleotide

• Length: 357 bp

>AT77535 NZ_CP021824:c342395-342039 [Sinorhizobium meliloti]
ATGAACACCATGACCTACAACGGGTATCACGCTCGCATCGAATTCGACGCTGAGGACGAAGTGTTTTTCGGCAAGATTGC
AGGTATCTCCGATGTAATCGGCTTTCACGGTGACAGTGTCGCTGAACTCAAGAAAGCCTTCCATGAGGCGGTCGATGACT
ACCTTGAGACCTGCAGGAAAATCGGCAAAGAGCCTCAGCGACCCTATTCTGGGAAGATGATGTTTCGTGTCGCTCCCGAA
GTCCATCGCCGAGCAGCTCTTGCTGCTGAGCTTTCGGGAAAGAGTCTCAACCAGTGGGCAGAAGAGGTGCTGGAAGAAGC
CGCCGGCCATTTTGCCGAGGCGCGCCTGTCGGCGTAG