Detailed information of TA system TA077533

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 46775..47355
• Replicon: plasmid psymA
• Accession: NZ_CP021824
• Organism: Sinorhizobium meliloti strain KH46

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q92XP1
• Locus tag: CDO24_RS26660
• Protein ID: WP_010968151.1
• Coordinates: 46775..47158 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CDO24_RS26665
• Protein ID: WP_088199999.1
• Coordinates: 47155..47355 (-)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO24_RS26640	42015..42950	-	936	WP_088199998.1	LysR family transcriptional regulator	-
CDO24_RS26650	43947..45998	+	2052	Protein_40	S9 family peptidase	-
CDO24_RS26655	46219..46503	+	285	WP_100208865.1	hypothetical protein	-
CDO24_RS26660	46775..47158	-	384	WP_010968151.1	type II toxin-antitoxin system VapC family toxin	Toxin
CDO24_RS26665	47155..47355	-	201	WP_088199999.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CDO24_RS26670	47646..48047	+	402	WP_014531161.1	GFA family protein	-
CDO24_RS26675	48124..48960	-	837	WP_014531162.1	helix-turn-helix transcriptional regulator	-
CDO24_RS26680	49056..49379	+	324	WP_088200000.1	DUF1127 domain-containing protein	-
CDO24_RS26685	49418..50146	+	729	WP_014531163.1	class I SAM-dependent methyltransferase	-
CDO24_RS26690	50240..50386	+	147	WP_017269032.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..1597380	1597380

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-113)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14292.54 Da
• Isoelectric Point: 6.9172

>T77533 WP_010968151.1 NZ_CP021824:c47158-46775 [Sinorhizobium meliloti]
VILADTSIWIDHFRHTDAELRRIIEDDRLLCHPAVIGELALGSLRERSSVIAFLMAQREALVATHQEVMMMIDRHAIFSM
GIGYTDAHLLASVLLDQRMALWTRDKRLQAAAEKAGASLHTPAHTRN

Nucleotide

• Length: 384 bp

>T77533 NZ_CP021824:c47158-46775 [Sinorhizobium meliloti]
GTGATTCTTGCCGACACTTCAATCTGGATTGATCATTTCCGGCATACCGATGCCGAGCTGCGTAGGATTATTGAGGACGA
TCGTCTCCTCTGCCATCCGGCCGTGATCGGAGAGCTTGCACTTGGCAGCCTTCGGGAACGCAGTAGCGTGATAGCATTCC
TGATGGCTCAGCGCGAAGCACTCGTCGCAACGCACCAGGAGGTCATGATGATGATCGATCGCCACGCCATTTTTAGCATG
GGGATTGGCTACACGGATGCCCATTTGCTGGCCTCGGTTCTCCTCGATCAGCGAATGGCTTTGTGGACGAGAGACAAGCG
TTTGCAGGCAGCGGCCGAAAAGGCGGGGGCGTCGCTGCACACCCCCGCCCATACGCGGAACTGA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7080.22 Da
• Isoelectric Point: 11.1107

>AT77533 WP_088199999.1 NZ_CP021824:c47355-47155 [Sinorhizobium meliloti]
MRSTINLDDTLMERAKALTGTKETAALVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Nucleotide

• Length: 201 bp

>AT77533 NZ_CP021824:c47355-47155 [Sinorhizobium meliloti]
ATGCGATCGACTATCAACCTCGACGACACTCTCATGGAAAGGGCTAAGGCCCTAACCGGCACAAAAGAAACCGCCGCTCT
GGTCCGCCAGGCATTGGAGACGCTTGTTCGTGTGGAGTCAGGAAAACGCCTCATCGCGCTCGGGGGAACTATGCCGGATG
CGGAAGCAGCTCCACGCCGCCGGAGCGCAGCTGCCAAGTGA

Structures

Toxin

• AlphaFold DB: Q92XP1