Detailed information of TA system TA077530

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3645537..3646168
• Replicon: chromosome
• Accession: NZ_CP021822
• Organism: Sinorhizobium meliloti strain KH46

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CDO24_RS17985
• Protein ID: WP_013844828.1
• Coordinates: 3645537..3645935 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q92MD9
• Locus tag: CDO24_RS17990
• Protein ID: WP_010970129.1
• Coordinates: 3645935..3646168 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO24_RS17950	3640619..3640921	-	303	WP_010970122.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CDO24_RS17955	3640918..3641193	-	276	WP_010970123.1	type II toxin-antitoxin system ParD family antitoxin	-
CDO24_RS17960	3641479..3641628	+	150	WP_010970124.1	hypothetical protein	-
CDO24_RS17965	3641625..3642560	+	936	WP_010970125.1	site-specific tyrosine recombinase XerD	-
CDO24_RS17970	3642624..3643577	+	954	WP_003527479.1	acetyl-CoA carboxylase carboxyltransferase subunit alpha	-
CDO24_RS17975	3643822..3645264	+	1443	WP_010970126.1	murein L,D-transpeptidase	-
CDO24_RS17980	3645264..3645503	+	240	WP_010970127.1	sulfurtransferase TusA family protein	-
CDO24_RS17985	3645537..3645935	-	399	WP_013844828.1	type II toxin-antitoxin system toxin VapC	Toxin
CDO24_RS17990	3645935..3646168	-	234	WP_010970129.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CDO24_RS17995	3646277..3647422	-	1146	WP_010970130.1	GTP-binding protein	-
CDO24_RS18000	3647436..3648518	-	1083	WP_010970131.1	D-alanyl-D-alanine carboxypeptidase	-
CDO24_RS18005	3648770..3649933	+	1164	WP_010970132.1	amidohydrolase	-
CDO24_RS18010	3649956..3650420	+	465	WP_010970133.1	Lrp/AsnC ligand binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (7-56)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14829.13 Da
• Isoelectric Point: 6.9581

>T77530 WP_013844828.1 NZ_CP021822:c3645935-3645537 [Sinorhizobium meliloti]
MLTYMLDTNICIYVMKTYPPAVREKFNGLAEQLCISSITLGELHYGAEKSARRVENLTAIEHFVARLEVLPFADKAAAHY
GQVRAELERTGTPCGPHDMQIGAHARSEGLIVVTNNIREFVRMPGVRVENWL

Nucleotide

• Length: 399 bp

>T77530 NZ_CP021822:c3645935-3645537 [Sinorhizobium meliloti]
ATGCTCACCTATATGCTCGATACCAATATCTGCATCTATGTGATGAAGACCTATCCGCCGGCCGTGCGCGAGAAGTTCAA
CGGACTGGCGGAGCAGCTTTGCATATCGAGCATCACGCTGGGAGAATTACACTATGGCGCCGAAAAATCGGCCCGGCGCG
TTGAGAACCTTACGGCTATCGAACATTTCGTGGCACGGCTGGAGGTGCTGCCGTTCGCAGACAAGGCAGCGGCCCACTAC
GGGCAGGTTCGGGCTGAACTGGAGCGGACGGGAACGCCATGCGGCCCTCATGACATGCAGATCGGCGCGCATGCGCGCAG
CGAAGGGCTGATTGTCGTGACCAACAATATACGCGAGTTCGTCCGCATGCCGGGTGTAAGGGTCGAGAACTGGCTGTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8673.87 Da
• Isoelectric Point: 8.9167

>AT77530 WP_010970129.1 NZ_CP021822:c3646168-3645935 [Sinorhizobium meliloti]
MASSTVFISNRSQAVRLPKAVAFPEGVHQVDILKIGRSRVIVPQGKRWDDLFLSGPRVSEDFMSERDQPVAETRESF

Nucleotide

• Length: 234 bp

>AT77530 NZ_CP021822:c3646168-3645935 [Sinorhizobium meliloti]
TTGGCCAGTTCGACCGTATTCATCAGTAACCGTAGCCAGGCGGTGCGGCTGCCAAAGGCCGTCGCCTTTCCCGAGGGCGT
GCATCAGGTCGATATCCTCAAGATCGGCCGCAGCCGCGTGATCGTCCCCCAGGGCAAAAGATGGGACGATCTGTTCCTCA
GCGGGCCGCGCGTCAGCGAGGATTTCATGAGCGAGCGTGATCAGCCGGTGGCGGAAACACGCGAATCCTTCTGA

Structures

Antitoxin

• AlphaFold DB: Q92MD9