77529

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacT-ataR/DUF1778(antitoxin)
Location 3597990..3598756 Replicon chromosome
Accession NZ_CP021822
Organism Sinorhizobium meliloti strain KH46

Toxin (Protein)


Gene name tacT Uniprot ID H0G6D3
Locus tag CDO24_RS17720 Protein ID WP_003533488.1
Coordinates 3598274..3598756 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID H0G6D4
Locus tag CDO24_RS17715 Protein ID WP_003533490.1
Coordinates 3597990..3598274 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDO24_RS17700 3593460..3594848 + 1389 WP_003533497.1 lipopolysaccharide biosynthesis protein -
CDO24_RS17705 3594852..3596123 + 1272 WP_010970087.1 GNAT family N-acetyltransferase -
CDO24_RS17710 3596139..3597785 - 1647 WP_088199571.1 AMP-binding protein -
CDO24_RS17715 3597990..3598274 + 285 WP_003533490.1 DUF1778 domain-containing protein Antitoxin
CDO24_RS17720 3598274..3598756 + 483 WP_003533488.1 GNAT family N-acetyltransferase Toxin
CDO24_RS17730 3599221..3599730 + 510 WP_013844819.1 disulfide bond formation protein B -
CDO24_RS17735 3599762..3600319 - 558 WP_003533486.1 HNH endonuclease -
CDO24_RS17740 3600425..3601072 - 648 WP_010970091.1 DNA-3-methyladenine glycosylase -
CDO24_RS17745 3601225..3602121 + 897 WP_010970092.1 tRNA glutamyl-Q(34) synthetase GluQRS -
CDO24_RS17750 3602140..3603015 - 876 WP_010970093.1 YihY/virulence factor BrkB family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(42-142)

Antitoxin

Vibrio phage ICP1, Orf50

(1-92)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17463.88 Da        Isoelectric Point: 7.3827

>T77529 WP_003533488.1 NZ_CP021822:3598274-3598756 [Sinorhizobium meliloti]
MLSAPTPLGEAHDFDLFQSGNDTLDDWLRRRAHANQASGASRTYVIAEEWRVVGYYCLASGALDLADAPSSVRRNMPDPI
PMAVLGRLAIDRDWQGKGLGAALLQDAVLRSSQAADIMGIRGLLVHAISGEAKAFYEHYGFQCSPNHPMTLVLSLKGKRR

Download         Length: 483 bp

>T77529 NZ_CP021822:3598274-3598756 [Sinorhizobium meliloti]
ATGCTGTCGGCGCCGACGCCTCTTGGTGAAGCCCACGACTTCGATCTCTTCCAGAGCGGCAACGACACGCTCGATGACTG
GCTGCGCCGCCGTGCGCACGCTAATCAGGCCAGTGGCGCGTCGCGCACCTATGTTATCGCTGAAGAATGGCGCGTGGTCG
GCTATTACTGCCTTGCATCCGGTGCCCTCGATCTGGCCGACGCGCCGAGCTCTGTCCGGCGCAACATGCCGGATCCCATC
CCGATGGCGGTGCTCGGTCGCCTTGCTATCGACCGCGACTGGCAGGGCAAAGGGTTGGGCGCGGCGCTATTGCAAGATGC
CGTCCTGCGGTCCTCGCAGGCGGCCGACATCATGGGAATACGGGGCTTGCTCGTGCATGCCATCTCGGGCGAGGCGAAAG
CCTTTTACGAACATTACGGCTTCCAGTGCTCACCCAACCATCCCATGACGCTGGTGTTGTCGCTCAAGGGCAAGCGCCGT
TAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10771.12 Da        Isoelectric Point: 7.3175

>AT77529 WP_003533490.1 NZ_CP021822:3597990-3598274 [Sinorhizobium meliloti]
MATKSSTDTHAKPVNLRIREDIRKVIDRAASIRGKTRSDFMIDAAYRAAEDTLLDQTLVRVDEHSYRHYLDILDQPPSGD
GFERLMNAPKPWQS

Download         Length: 285 bp

>AT77529 NZ_CP021822:3597990-3598274 [Sinorhizobium meliloti]
ATGGCCACGAAATCGTCGACTGACACCCACGCGAAGCCCGTCAACCTGCGAATCCGAGAGGATATACGCAAGGTGATCGA
TCGCGCCGCGAGCATTCGCGGCAAGACCCGCTCCGATTTTATGATCGACGCAGCCTACCGCGCGGCCGAAGACACGCTGC
TCGATCAGACGCTGGTGCGGGTCGATGAGCACAGCTATCGGCACTACCTAGACATTCTTGACCAGCCGCCAAGTGGCGAC
GGCTTCGAACGCCTGATGAATGCGCCGAAACCCTGGCAGTCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB H0G6D3

Antitoxin

Source ID Structure
AlphaFold DB H0G6D4

References