Detailed information of TA system TA077526

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-RHH
• Location: 2019292..2019866
• Replicon: chromosome
• Accession: NZ_CP021822
• Organism: Sinorhizobium meliloti strain KH46

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q92QN4
• Locus tag: CDO24_RS09625
• Protein ID: WP_010969154.1
• Coordinates: 2019292..2019657 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q92QN3
• Locus tag: CDO24_RS09630
• Protein ID: WP_010969155.1
• Coordinates: 2019651..2019866 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO24_RS09600	2014432..2015874	+	1443	WP_010969150.1	NADH-quinone oxidoreductase subunit NuoN	-
CDO24_RS09605	2015871..2016638	+	768	WP_003531861.1	biotin--[acetyl-CoA-carboxylase] ligase	-
CDO24_RS09610	2016666..2018333	+	1668	WP_010969151.1	ribonuclease J	-
CDO24_RS09615	2018392..2018796	+	405	WP_010969152.1	methylmalonyl-CoA epimerase	-
CDO24_RS09620	2018936..2019205	+	270	WP_014526683.1	DUF1467 family protein	-
CDO24_RS09625	2019292..2019657	-	366	WP_010969154.1	type II toxin-antitoxin system VapC family toxin	Toxin
CDO24_RS09630	2019651..2019866	-	216	WP_010969155.1	ribbon-helix-helix protein, CopG family	Antitoxin
CDO24_RS09635	2019934..2020161	-	228	WP_088199345.1	hypothetical protein	-
CDO24_RS09640	2020577..2021905	+	1329	WP_088199346.1	proline--tRNA ligase	-
CDO24_RS09645	2021926..2023236	+	1311	WP_014529722.1	lipoprotein-releasing ABC transporter permease subunit	-
CDO24_RS09650	2023254..2023940	+	687	WP_010969157.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-106)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-40)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13592.57 Da
• Isoelectric Point: 5.1787

>T77526 WP_010969154.1 NZ_CP021822:c2019657-2019292 [Sinorhizobium meliloti]
MVGALFDTNILIDHLNAVPQAHKELDRFENRAISIITWMEVMVGADAELVEPTRRFLDGFETIALNDEIANRAVTLRRAH
RIKLPDAVIWATAQTAGRLLVTRNTKDFPADDPGIREPYAV

Nucleotide

• Length: 366 bp

>T77526 NZ_CP021822:c2019657-2019292 [Sinorhizobium meliloti]
ATGGTAGGCGCACTGTTCGATACCAATATCCTTATCGATCATCTGAATGCGGTGCCTCAGGCGCACAAGGAACTTGACCG
TTTCGAAAACCGGGCCATCAGCATCATCACCTGGATGGAGGTGATGGTTGGAGCGGACGCCGAACTGGTTGAGCCGACCC
GGCGCTTCCTCGACGGTTTCGAAACGATCGCCTTGAACGATGAGATCGCCAACCGTGCGGTGACATTGCGCAGGGCGCAC
CGCATCAAGCTACCCGACGCGGTGATCTGGGCAACGGCGCAGACTGCCGGTCGTCTGCTCGTGACGCGCAATACCAAGGA
CTTTCCAGCCGACGACCCGGGGATACGCGAACCCTACGCCGTGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8381.51 Da
• Isoelectric Point: 8.6052

>AT77526 WP_010969155.1 NZ_CP021822:c2019866-2019651 [Sinorhizobium meliloti]
MRALIDMNDTQVEALDTLAKRVRRSRAALIREAIDDYLNRHHREQIEDGFGLWGKRKVDGLAYQEKVRGEW

Nucleotide

• Length: 216 bp

>AT77526 NZ_CP021822:c2019866-2019651 [Sinorhizobium meliloti]
ATGCGAGCCTTGATCGATATGAACGATACCCAGGTCGAAGCCCTAGATACCCTCGCCAAGCGGGTGCGCCGGTCCCGTGC
GGCTCTGATCCGCGAAGCGATCGACGACTATCTCAACCGTCATCATCGCGAGCAGATCGAAGATGGCTTCGGTTTGTGGG
GCAAGCGCAAAGTTGACGGTCTGGCTTATCAGGAGAAGGTGCGCGGCGAATGGTAG

Structures

Toxin

• AlphaFold DB: Q92QN4

Antitoxin

• AlphaFold DB: Q92QN3