77526

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-RHH
Location 2019292..2019866 Replicon chromosome
Accession NZ_CP021822
Organism Sinorhizobium meliloti strain KH46

Toxin (Protein)


Gene name vapC Uniprot ID Q92QN4
Locus tag CDO24_RS09625 Protein ID WP_010969154.1
Coordinates 2019292..2019657 (-) Length 122 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q92QN3
Locus tag CDO24_RS09630 Protein ID WP_010969155.1
Coordinates 2019651..2019866 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDO24_RS09600 2014432..2015874 + 1443 WP_010969150.1 NADH-quinone oxidoreductase subunit NuoN -
CDO24_RS09605 2015871..2016638 + 768 WP_003531861.1 biotin--[acetyl-CoA-carboxylase] ligase -
CDO24_RS09610 2016666..2018333 + 1668 WP_010969151.1 ribonuclease J -
CDO24_RS09615 2018392..2018796 + 405 WP_010969152.1 methylmalonyl-CoA epimerase -
CDO24_RS09620 2018936..2019205 + 270 WP_014526683.1 DUF1467 family protein -
CDO24_RS09625 2019292..2019657 - 366 WP_010969154.1 type II toxin-antitoxin system VapC family toxin Toxin
CDO24_RS09630 2019651..2019866 - 216 WP_010969155.1 ribbon-helix-helix protein, CopG family Antitoxin
CDO24_RS09635 2019934..2020161 - 228 WP_088199345.1 hypothetical protein -
CDO24_RS09640 2020577..2021905 + 1329 WP_088199346.1 proline--tRNA ligase -
CDO24_RS09645 2021926..2023236 + 1311 WP_014529722.1 lipoprotein-releasing ABC transporter permease subunit -
CDO24_RS09650 2023254..2023940 + 687 WP_010969157.1 ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-106)

Antitoxin

Ribbon-helix-helix protein, CopG

(9-40)


Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 13592.57 Da        Isoelectric Point: 5.1787

>T77526 WP_010969154.1 NZ_CP021822:c2019657-2019292 [Sinorhizobium meliloti]
MVGALFDTNILIDHLNAVPQAHKELDRFENRAISIITWMEVMVGADAELVEPTRRFLDGFETIALNDEIANRAVTLRRAH
RIKLPDAVIWATAQTAGRLLVTRNTKDFPADDPGIREPYAV

Download         Length: 366 bp

>T77526 NZ_CP021822:c2019657-2019292 [Sinorhizobium meliloti]
ATGGTAGGCGCACTGTTCGATACCAATATCCTTATCGATCATCTGAATGCGGTGCCTCAGGCGCACAAGGAACTTGACCG
TTTCGAAAACCGGGCCATCAGCATCATCACCTGGATGGAGGTGATGGTTGGAGCGGACGCCGAACTGGTTGAGCCGACCC
GGCGCTTCCTCGACGGTTTCGAAACGATCGCCTTGAACGATGAGATCGCCAACCGTGCGGTGACATTGCGCAGGGCGCAC
CGCATCAAGCTACCCGACGCGGTGATCTGGGCAACGGCGCAGACTGCCGGTCGTCTGCTCGTGACGCGCAATACCAAGGA
CTTTCCAGCCGACGACCCGGGGATACGCGAACCCTACGCCGTGTAG

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 8381.51 Da        Isoelectric Point: 8.6052

>AT77526 WP_010969155.1 NZ_CP021822:c2019866-2019651 [Sinorhizobium meliloti]
MRALIDMNDTQVEALDTLAKRVRRSRAALIREAIDDYLNRHHREQIEDGFGLWGKRKVDGLAYQEKVRGEW

Download         Length: 216 bp

>AT77526 NZ_CP021822:c2019866-2019651 [Sinorhizobium meliloti]
ATGCGAGCCTTGATCGATATGAACGATACCCAGGTCGAAGCCCTAGATACCCTCGCCAAGCGGGTGCGCCGGTCCCGTGC
GGCTCTGATCCGCGAAGCGATCGACGACTATCTCAACCGTCATCATCGCGAGCAGATCGAAGATGGCTTCGGTTTGTGGG
GCAAGCGCAAAGTTGACGGTCTGGCTTATCAGGAGAAGGTGCGCGGCGAATGGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q92QN4

Antitoxin

Source ID Structure
AlphaFold DB Q92QN3

References