Detailed information of TA system TA077523

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 1302927..1303504
• Replicon: chromosome
• Accession: NZ_CP021822
• Organism: Sinorhizobium meliloti strain KH46

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: Q92S20
• Locus tag: CDO24_RS06085
• Protein ID: WP_010968707.1
• Coordinates: 1302927..1303190 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q92KN5
• Locus tag: CDO24_RS06090
• Protein ID: WP_010968708.1
• Coordinates: 1303187..1303504 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO24_RS06045	1298256..1298696	+	441	WP_010968702.1	DUF2948 family protein	-
CDO24_RS06050	1298761..1300065	+	1305	WP_010968703.1	histidinol dehydrogenase	-
CDO24_RS06055	1300062..1300538	+	477	WP_010968704.1	UPF0262 family protein	-
CDO24_RS06060	1300552..1301031	+	480	WP_010968705.1	low molecular weight phosphatase family protein	-
CDO24_RS06065	1301189..1301407	+	219	WP_004435948.1	translation initiation factor IF-1	-
CDO24_RS06070	1301505..1302125	+	621	WP_004435951.1	Maf-like protein	-
CDO24_RS06075	1302130..1302342	+	213	WP_010968706.1	DNA gyrase inhibitor YacG	-
CDO24_RS06085	1302927..1303190	+	264	WP_010968707.1	BrnT family toxin	Toxin
CDO24_RS06090	1303187..1303504	+	318	WP_010968708.1	BrnA antitoxin family protein	Antitoxin
CDO24_RS06095	1303707..1304171	-	465	WP_013844019.1	type II toxin-antitoxin system HicB family antitoxin	-
CDO24_RS06100	1304168..1304356	-	189	WP_013844020.1	hypothetical protein	-
CDO24_RS06105	1304676..1305776	+	1101	WP_010968710.1	hypothetical protein	-
CDO24_RS06110	1305763..1306845	+	1083	WP_010968711.1	nucleotidyltransferase family protein	-
CDO24_RS06115	1307048..1307359	+	312	WP_004435964.1	hypothetical protein	-
CDO24_RS06120	1307639..1308028	-	390	WP_013844021.1	DUF930 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1277122..1304356	27234

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-82)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (26-103)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 9821.16 Da
• Isoelectric Point: 10.3413

>T77523 WP_010968707.1 NZ_CP021822:1302927-1303190 [Sinorhizobium meliloti]
MRIVWGEPKRRANLEKHGFDFADVSDLDWASALIEDSKPDTSGRKRLKAIGYFRDGTAAVIFATLGTEAISIISFRPASD
RERRRLP

Nucleotide

• Length: 264 bp

>T77523 NZ_CP021822:1302927-1303190 [Sinorhizobium meliloti]
ATGCGAATTGTGTGGGGCGAACCCAAACGGCGAGCCAACCTTGAGAAGCATGGTTTCGATTTTGCCGATGTAAGCGACCT
CGATTGGGCGAGTGCCCTCATAGAGGACAGCAAGCCAGATACTTCTGGCAGAAAGCGCCTGAAGGCTATCGGTTATTTTC
GTGACGGGACGGCAGCGGTCATTTTCGCGACACTCGGAACGGAAGCTATATCGATCATCAGCTTTCGTCCTGCAAGCGAC
AGAGAAAGAAGGAGGCTTCCATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12197.83 Da
• Isoelectric Point: 10.0883

>AT77523 WP_010968708.1 NZ_CP021822:1303187-1303504 [Sinorhizobium meliloti]
MTTRTRKSKTFEPGHGYTKEEWDAVDFPELTDEELTNMRPARDVLPPEFFKAMEEHRRSRGRPSLEHPKKQVTLRLDEDV
IAKFRAGGKGWQSRMNEALRKAAGI

Nucleotide

• Length: 318 bp

>AT77523 NZ_CP021822:1303187-1303504 [Sinorhizobium meliloti]
ATGACTACCAGGACCCGAAAATCGAAAACGTTCGAGCCAGGTCACGGCTATACAAAAGAGGAATGGGATGCAGTCGATTT
TCCCGAGTTGACCGATGAAGAGCTTACCAACATGCGTCCCGCAAGAGATGTGCTGCCGCCTGAGTTTTTCAAAGCTATGG
AGGAGCATCGCAGATCGCGTGGGCGCCCCTCGCTGGAGCATCCGAAAAAGCAAGTCACCCTCCGGTTGGACGAAGACGTG
ATCGCTAAGTTTCGAGCTGGTGGCAAAGGCTGGCAGAGCCGGATGAATGAGGCGCTGCGAAAAGCTGCAGGTATATAG

Structures

Toxin

• AlphaFold DB: Q92S20

Antitoxin

• AlphaFold DB: Q92KN5