Detailed information of TA system TA077522

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 591821..592482
• Replicon: chromosome
• Accession: NZ_CP021822
• Organism: Sinorhizobium meliloti strain KH46

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q92KY5
• Locus tag: CDO24_RS02710
• Protein ID: WP_010970565.1
• Coordinates: 591821..592225 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q92KY4
• Locus tag: CDO24_RS02715
• Protein ID: WP_010970566.1
• Coordinates: 592222..592482 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO24_RS02695	587364..588476	+	1113	WP_003535893.1	3-isopropylmalate dehydrogenase	-
CDO24_RS02700	588605..589312	+	708	WP_010970563.1	phosphatase PAP2 family protein	-
CDO24_RS02705	589413..591302	-	1890	WP_088199256.1	MHS family MFS transporter	-
CDO24_RS02710	591821..592225	-	405	WP_010970565.1	type II toxin-antitoxin system VapC family toxin	Toxin
CDO24_RS02715	592222..592482	-	261	WP_010970566.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CDO24_RS36165	592611..592781	+	171	WP_010970567.1	hypothetical protein	-
CDO24_RS02725	592895..593929	+	1035	WP_003531790.1	aspartate-semialdehyde dehydrogenase	-
CDO24_RS02730	594102..594923	+	822	WP_010970568.1	lytic transglycosylase family protein	-
CDO24_RS02740	595270..595953	-	684	WP_010970569.1	carbonic anhydrase	-
CDO24_RS02745	596127..597008	-	882	WP_010970570.1	pyridoxal kinase PdxY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (8-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14507.71 Da
• Isoelectric Point: 6.7257

>T77522 WP_010970565.1 NZ_CP021822:c592225-591821 [Sinorhizobium meliloti]
VISHILDTNAVIALIGRKSDALVTRVLHSPQGIIGLPSVVAYELYFGAQKSAKAQHNLETLRLLMADFPILDFDRNDAFV
AGEIRAALAAKGTPIGPYDVLIAGQAKARGLTLVTNNVGEFNRVENLRVEDWSL

Nucleotide

• Length: 405 bp

>T77522 NZ_CP021822:c592225-591821 [Sinorhizobium meliloti]
GTGATCTCCCATATTCTCGACACCAACGCCGTCATCGCTTTGATCGGGCGCAAATCGGATGCATTGGTCACCCGTGTACT
CCATAGCCCTCAGGGCATTATCGGGCTCCCGTCGGTCGTCGCGTATGAACTCTATTTTGGAGCGCAGAAAAGCGCGAAGG
CCCAACACAATCTCGAAACCCTGCGTTTGTTGATGGCGGATTTTCCGATCCTCGACTTCGACCGGAACGATGCCTTTGTC
GCAGGCGAGATCCGGGCCGCCCTTGCCGCGAAGGGTACGCCCATCGGACCTTATGACGTGCTGATTGCGGGACAGGCCAA
AGCCCGTGGATTGACGCTCGTCACCAACAATGTCGGCGAGTTCAATAGGGTTGAGAACCTGCGCGTGGAGGATTGGTCGC
TCTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9903.16 Da
• Isoelectric Point: 5.1253

>AT77522 WP_010970566.1 NZ_CP021822:c592482-592222 [Sinorhizobium meliloti]
MPHLARVFQSGNSQAVRLPKEFRFNVDRVEITQEGDALILRPHVEQGEQWSSLKAALARGMSEDFMMCGREQPEQQDRSE
LDAVFR

Nucleotide

• Length: 261 bp

>AT77522 NZ_CP021822:c592482-592222 [Sinorhizobium meliloti]
ATGCCCCATCTTGCACGGGTGTTCCAGTCCGGAAATTCGCAGGCCGTCCGCCTGCCTAAGGAATTCCGCTTCAACGTCGA
TCGCGTCGAAATCACGCAGGAAGGCGACGCGCTCATTCTCCGCCCGCACGTCGAGCAAGGCGAGCAGTGGTCATCGCTGA
AGGCGGCACTTGCCCGTGGCATGAGTGAAGACTTCATGATGTGCGGGCGCGAGCAGCCGGAACAGCAGGATCGGTCGGAA
CTCGACGCGGTATTTCGGTGA

Structures

Toxin

• AlphaFold DB: Q92KY5

Antitoxin

• AlphaFold DB: Q92KY4