Detailed information of TA system TA077521

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 332521..333056
• Replicon: plasmid accessoryA
• Accession: NZ_CP021821
• Organism: Sinorhizobium meliloti strain M162

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CDO25_RS33555
• Protein ID: WP_088201691.1
• Coordinates: 332521..332796 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CDO25_RS33560
• Protein ID: WP_088197958.1
• Coordinates: 332796..333056 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO25_RS33525	327722..329176	+	1455	WP_088201688.1	FAD-binding oxidoreductase	-
CDO25_RS33530	329173..330786	+	1614	WP_088201690.1	GMC family oxidoreductase N-terminal domain-containing protein	-
CDO25_RS33535	330928..331134	-	207	Protein_314	FAD-binding oxidoreductase	-
CDO25_RS34710	331075..331374	+	300	WP_153416893.1	hypothetical protein	-
CDO25_RS33555	332521..332796	-	276	WP_088201691.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDO25_RS33560	332796..333056	-	261	WP_088197958.1	ribbon-helix-helix domain-containing protein	Antitoxin
CDO25_RS33565	333228..333454	+	227	Protein_318	transposase	-
CDO25_RS33575	334511..335539	-	1029	WP_088201692.1	LLM class flavin-dependent oxidoreductase	-
CDO25_RS33580	335966..337081	-	1116	WP_088197961.1	histidinol-phosphate transaminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..416269	416269

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10867.49 Da
• Isoelectric Point: 10.8743

>T77521 WP_088201691.1 NZ_CP021821:c332796-332521 [Sinorhizobium meliloti]
MKLEWTRKAVSDLGRLHDFLAPVNRQAAARTVQSLTSAPARLLEQPRIGERLEEFEPREVRRILVGHYEIRYEIRQSTIY
VLRLWHTRESR

Nucleotide

• Length: 276 bp

>T77521 NZ_CP021821:c332796-332521 [Sinorhizobium meliloti]
ATGAAGCTCGAGTGGACGCGTAAGGCAGTCTCCGATCTAGGGCGCCTGCATGATTTCCTGGCACCGGTAAATCGGCAAGC
TGCTGCTCGCACTGTGCAATCGTTGACAAGCGCTCCTGCGCGCCTGCTCGAACAACCGCGCATCGGCGAACGGCTGGAGG
AATTCGAGCCACGAGAGGTTCGGCGAATTCTCGTTGGCCATTACGAAATACGGTATGAGATTCGGCAGTCGACGATCTAC
GTGCTGCGGCTTTGGCATACGCGTGAATCACGGTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9685.09 Da
• Isoelectric Point: 5.1200

>AT77521 WP_088197958.1 NZ_CP021821:c333056-332796 [Sinorhizobium meliloti]
METKVLTAHVPLPLAEKVDQIAARLERSRGWIVKQALTAWVDQEEERRRLTLEALADVDAGRVIEHHAVQAWADSLDSDK
PLPLPL

Nucleotide

• Length: 261 bp

>AT77521 NZ_CP021821:c333056-332796 [Sinorhizobium meliloti]
ATGGAAACCAAGGTGCTGACCGCGCACGTGCCACTGCCACTCGCCGAGAAAGTCGATCAGATCGCCGCCCGCCTTGAGCG
CTCGCGCGGGTGGATCGTCAAGCAGGCGCTGACCGCCTGGGTCGATCAGGAAGAGGAGCGCCGACGCTTGACGCTGGAAG
CGCTTGCCGACGTCGACGCTGGCCGCGTGATCGAGCACCATGCCGTTCAGGCCTGGGCCGACAGTCTTGACAGCGACAAG
CCGCTACCCTTGCCGCTGTGA