Detailed information of TA system TA077520

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 203467..204137
• Replicon: plasmid accessoryA
• Accession: NZ_CP021821
• Organism: Sinorhizobium meliloti strain M162

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CDO25_RS32880
• Protein ID: WP_088197815.1
• Coordinates: 203718..204137 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CDO25_RS32875
• Protein ID: WP_088197814.1
• Coordinates: 203467..203721 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO25_RS32855	199649..200584	+	936	WP_088197812.1	ABC transporter permease	-
CDO25_RS32860	200590..201411	+	822	WP_010967053.1	ABC transporter permease	-
CDO25_RS32865	201408..202322	+	915	WP_088197813.1	ABC transporter ATP-binding protein	-
CDO25_RS32870	202319..203281	+	963	WP_088201619.1	ABC transporter ATP-binding protein	-
CDO25_RS32875	203467..203721	+	255	WP_088197814.1	plasmid stabilization protein	Antitoxin
CDO25_RS32880	203718..204137	+	420	WP_088197815.1	type II toxin-antitoxin system VapC family toxin	Toxin
CDO25_RS32885	204106..204534	-	429	WP_153416919.1	GFA family protein	-
CDO25_RS32890	204937..207471	+	2535	WP_192936239.1	PAS domain S-box protein	-
CDO25_RS32895	207499..207813	+	315	Protein_188	response regulator	-
CDO25_RS32905	207879..209081	+	1203	WP_010967204.1	IS256-like element ISRm3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..416269	416269
	-	flank	IS/Tn	-	-	207879..209081	1202

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-125)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14885.21 Da
• Isoelectric Point: 6.4653

>T77520 WP_088197815.1 NZ_CP021821:203718-204137 [Sinorhizobium meliloti]
MIVLDTNVVSEAMKPAPDPAVRSWLNEQVAETLYLASVTLAELLFGIGALPAGRRKKALPEMLDGVLELFGDRVLPFDIG
AARCYAELQVTARAAGKGFPPPDGYIAAIAASRGFIVATRDSRPFEAAGLTVANPWHHR

Nucleotide

• Length: 420 bp

>T77520 NZ_CP021821:203718-204137 [Sinorhizobium meliloti]
ATGATTGTCCTGGATACGAATGTCGTCTCCGAGGCGATGAAACCGGCCCCCGATCCGGCCGTGCGCTCCTGGTTGAACGA
GCAGGTCGCTGAAACCTTGTATCTGGCCAGTGTGACCTTGGCCGAACTGCTGTTCGGCATCGGTGCGCTGCCGGCCGGTC
GGCGCAAGAAGGCTCTGCCCGAAATGCTTGACGGCGTGCTCGAACTGTTTGGCGATCGCGTATTGCCGTTCGACATTGGA
GCTGCTCGCTGCTATGCCGAGCTTCAGGTGACCGCTCGAGCGGCCGGCAAAGGATTTCCGCCGCCTGACGGATATATTGC
CGCCATTGCCGCCTCCAGGGGATTTATCGTCGCGACCCGCGACAGCAGACCGTTCGAGGCGGCTGGACTTACCGTGGCAA
ATCCTTGGCACCATCGATGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9439.78 Da
• Isoelectric Point: 6.4732

>AT77520 WP_088197814.1 NZ_CP021821:203467-203721 [Sinorhizobium meliloti]
MAILTGRNVPDEVHRALRVRAAMHGRSTEAEVREILESAVKPEPRVRMGDALAELGRRLGLTNDDFAVFDEVRDKAPAQP
MRFE

Nucleotide

• Length: 255 bp

>AT77520 NZ_CP021821:203467-203721 [Sinorhizobium meliloti]
ATGGCGATCCTGACGGGTCGGAATGTGCCCGATGAAGTGCATCGCGCCTTGCGCGTGAGAGCGGCGATGCACGGCCGCAG
CACCGAGGCGGAAGTCCGGGAGATTTTGGAAAGTGCGGTGAAGCCCGAGCCGCGCGTTCGCATGGGCGATGCCCTGGCCG
AGCTCGGCCGGCGACTGGGGCTGACCAATGACGATTTCGCAGTGTTCGACGAGGTACGTGACAAGGCGCCGGCGCAGCCA
ATGAGATTTGAATGA