Detailed information of TA system TA077519

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 1658250..1658917
• Replicon: plasmid psymB
• Accession: NZ_CP021820
• Organism: Sinorhizobium meliloti strain M162

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A8B3N559
• Locus tag: CDO25_RS31725
• Protein ID: WP_017266625.1
• Coordinates: 1658474..1658917 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CDO25_RS31720
• Protein ID: WP_088201529.1
• Coordinates: 1658250..1658477 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO25_RS31695	1653644..1654525	-	882	WP_003525869.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
CDO25_RS31700	1654643..1655935	-	1293	WP_014527485.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
CDO25_RS31710	1656304..1657593	+	1290	WP_088201505.1	SDR family oxidoreductase	-
CDO25_RS31715	1657593..1658066	+	474	WP_017266624.1	DUF2269 family protein	-
CDO25_RS31720	1658250..1658477	+	228	WP_088201529.1	CopG family transcriptional regulator	Antitoxin
CDO25_RS31725	1658474..1658917	+	444	WP_017266625.1	PIN domain-containing protein	Toxin
CDO25_RS31730	1658933..1659871	-	939	WP_013849980.1	choline ABC transporter substrate-binding protein	-
CDO25_RS31735	1659940..1660770	-	831	WP_013849981.1	SDR family oxidoreductase	-
CDO25_RS31740	1660767..1661264	-	498	WP_088201506.1	acetone carboxylase subunit gamma	-
CDO25_RS31745	1661268..1663460	-	2193	WP_088201507.1	hydantoinase B/oxoprolinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	htpB	1..1681904	1681904

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-131)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 15638.95 Da
• Isoelectric Point: 6.6378

>T77519 WP_017266625.1 NZ_CP021820:1658474-1658917 [Sinorhizobium meliloti]
VTFLLDVNVLIALIDPSHIGHDDAHEWFASMGQTAWATCPITENGVIRIVGNPEYPNSPGSPLPVMEIVAKLRSLPGHVF
WPDDVSLVGSSDIIPSKILTSGQVTDTYLLALAKARGGKLATFDRKLSAAAVTKGNSALHLIATNRS

Nucleotide

• Length: 444 bp

>T77519 NZ_CP021820:1658474-1658917 [Sinorhizobium meliloti]
GTGACTTTCCTGCTCGACGTCAATGTGCTGATTGCCTTGATCGATCCGAGCCATATAGGCCATGATGATGCGCACGAATG
GTTCGCGTCGATGGGTCAGACCGCGTGGGCCACCTGCCCAATTACCGAAAATGGTGTCATCCGGATCGTCGGCAATCCCG
AATATCCCAATTCTCCCGGCTCACCGTTACCCGTGATGGAGATCGTCGCCAAATTGCGGTCGCTCCCCGGTCATGTTTTT
TGGCCGGACGATGTGAGCCTTGTCGGGTCAAGTGATATCATCCCATCGAAAATCCTGACTTCGGGGCAGGTGACTGACAC
CTACCTGCTTGCGCTCGCCAAGGCGCGTGGGGGCAAGCTGGCGACATTCGATCGCAAGCTGTCGGCAGCGGCGGTCACGA
AGGGCAATTCCGCCTTGCACCTGATCGCGACGAATAGGTCGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8160.37 Da
• Isoelectric Point: 9.1096

>AT77519 WP_088201529.1 NZ_CP021820:1658250-1658477 [Sinorhizobium meliloti]
MRTTLAIDDDVLVAAKAMATQQQRSVGEVISELARRSLRRPRSGGERNGIPLLSPRPDAPPVTLEIVNALRDELA

Nucleotide

• Length: 228 bp

>AT77519 NZ_CP021820:1658250-1658477 [Sinorhizobium meliloti]
ATGAGAACGACCTTGGCTATTGACGACGATGTCCTGGTCGCCGCAAAGGCAATGGCGACCCAACAGCAGCGGAGCGTCGG
CGAGGTAATTTCCGAACTGGCGAGGCGTTCTCTGCGCCGGCCGCGCAGCGGCGGCGAGCGTAATGGAATTCCTCTCCTGT
CGCCTCGTCCGGACGCACCGCCCGTGACGCTCGAGATCGTCAACGCCCTGCGTGACGAACTGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A8B3N559