Detailed information of TA system TA077518

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-Xre
• Location: 1307560..1308492
• Replicon: plasmid psymB
• Accession: NZ_CP021820
• Organism: Sinorhizobium meliloti strain M162

Toxin (Protein)

• Gene name: -
• Uniprot ID: F7XGP1
• Locus tag: CDO25_RS29935
• Protein ID: WP_014530511.1
• Coordinates: 1307560..1308114 (-)
• Length: 185 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: F7XGP2
• Locus tag: CDO25_RS29940
• Protein ID: WP_014530512.1
• Coordinates: 1308121..1308492 (-)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO25_RS29915	1303089..1303955	+	867	WP_015008365.1	sulfate ABC transporter permease subunit CysT	-
CDO25_RS29920	1303945..1304820	+	876	WP_013850796.1	sulfate ABC transporter permease subunit CysW	-
CDO25_RS29925	1304836..1305942	+	1107	WP_013850797.1	sulfate/molybdate ABC transporter ATP-binding protein	-
CDO25_RS29930	1306054..1307424	+	1371	WP_015008367.1	FAD-binding oxidoreductase	-
CDO25_RS29935	1307560..1308114	-	555	WP_014530511.1	RES family NAD+ phosphorylase	Toxin
CDO25_RS29940	1308121..1308492	-	372	WP_014530512.1	DUF2384 domain-containing protein	Antitoxin
CDO25_RS29945	1308569..1309354	-	786	WP_014530513.1	crotonase/enoyl-CoA hydratase family protein	-
CDO25_RS29950	1309351..1310220	-	870	WP_014530514.1	hydroxymethylglutaryl-CoA lyase	-
CDO25_RS29955	1310217..1312205	-	1989	WP_014530515.1	acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha	-
CDO25_RS29960	1312222..1312605	-	384	WP_010975573.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	htpB	1..1681904	1681904

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (23-177)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (71-120)

Sequences

Toxin

Protein

• Length: 185 a.a.
• Molecular weight: 20449.31 Da
• Isoelectric Point: 6.4901

>T77518 WP_014530511.1 NZ_CP021820:c1308114-1307560 [Sinorhizobium meliloti]
MRKHIPPASRPDSEPTSPVEPITLWRAFVPRWAHMPLSGAGAARFGGRWNPIGMPAIYAARELSTAWAEYNQGFVQHPAL
IVQLELRGARLADLTDASVLLELGVDEAIHRCEWRDALDKGAVPETHRLQSELLARDYHGVIYPSFMSPGGTCVALWRWN
GAEEPRLDVIDPDGRLPKSPASWV

Nucleotide

• Length: 555 bp

>T77518 NZ_CP021820:c1308114-1307560 [Sinorhizobium meliloti]
ATGAGGAAACACATTCCGCCCGCATCGCGACCCGATTCGGAACCGACGTCGCCGGTCGAGCCGATAACGCTCTGGCGCGC
CTTCGTTCCGCGCTGGGCACATATGCCGCTATCCGGCGCGGGTGCCGCTCGCTTCGGTGGCCGCTGGAACCCGATCGGCA
TGCCTGCGATTTATGCTGCGCGCGAGCTTTCCACTGCCTGGGCTGAATATAATCAGGGTTTCGTCCAGCATCCGGCGCTC
ATCGTGCAGCTCGAATTGCGCGGGGCGAGGCTCGCGGACCTCACGGATGCCAGCGTTCTTCTGGAGCTTGGTGTGGATGA
GGCGATCCATCGCTGCGAATGGCGTGATGCGCTCGACAAAGGCGCGGTGCCGGAGACCCACAGGCTGCAGTCGGAGCTTC
TGGCGCGCGACTATCACGGGGTGATCTATCCATCCTTCATGTCGCCCGGCGGCACCTGCGTCGCGCTCTGGCGCTGGAAC
GGTGCGGAAGAACCGCGTCTCGACGTCATCGATCCCGATGGCCGGCTGCCGAAATCGCCCGCCTCATGGGTTTGA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13117.25 Da
• Isoelectric Point: 9.6233

>AT77518 WP_014530512.1 NZ_CP021820:c1308492-1308121 [Sinorhizobium meliloti]
MMAVDFQIAAARFGDDHSPFLSARLVADWLGVTLAELAKLIGVARNTLTAKSGARKVDSALSKVVRILAMASEMAGDEAR
AVIWFKHQPIPGWAGKTAFDLVGEGKADKVLAYLESVRAGVYA

Nucleotide

• Length: 372 bp

>AT77518 NZ_CP021820:c1308492-1308121 [Sinorhizobium meliloti]
ATGATGGCCGTTGATTTCCAGATAGCTGCAGCCCGTTTCGGGGACGATCATTCCCCGTTTCTCTCGGCCCGCCTTGTCGC
CGACTGGTTGGGCGTCACGCTCGCCGAACTCGCCAAGCTGATCGGTGTCGCCCGCAATACGCTGACGGCGAAATCCGGCG
CCCGGAAGGTCGACAGCGCCTTGAGCAAGGTCGTCCGCATCCTCGCCATGGCTTCCGAAATGGCGGGCGACGAAGCCCGC
GCCGTCATCTGGTTCAAGCACCAGCCTATCCCCGGCTGGGCCGGCAAGACGGCGTTCGATCTGGTCGGCGAGGGCAAGGC
CGACAAGGTGCTCGCCTATCTCGAATCGGTACGTGCCGGCGTCTACGCTTAA

Structures

Toxin

• AlphaFold DB: A0A222K2B4

Antitoxin

• AlphaFold DB: A0A222K374