Detailed information of TA system TA077517

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 315657..316191
• Replicon: plasmid psymB
• Accession: NZ_CP021820
• Organism: Sinorhizobium meliloti strain M162

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q92X99
• Locus tag: CDO25_RS25185
• Protein ID: WP_010974986.1
• Coordinates: 315657..315953 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: H0FXK0
• Locus tag: CDO25_RS25190
• Protein ID: WP_003527850.1
• Coordinates: 315943..316191 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO25_RS25155	311101..312504	-	1404	WP_088201280.1	sodium:alanine symporter family protein	-
CDO25_RS25160	313038..313247	-	210	WP_003527838.1	dodecin family protein	-
CDO25_RS25165	313537..313983	+	447	WP_041170054.1	DNA-binding protein	-
CDO25_RS25170	313987..314172	-	186	WP_010974989.1	hypothetical protein	-
CDO25_RS25175	314339..314737	-	399	WP_088201281.1	hypothetical protein	-
CDO25_RS25180	315316..315576	+	261	Protein_304	helix-turn-helix domain containing protein	-
CDO25_RS25185	315657..315953	-	297	WP_010974986.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDO25_RS25190	315943..316191	-	249	WP_003527850.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
CDO25_RS25195	316299..316454	-	156	WP_010974985.1	hypothetical protein	-
CDO25_RS25205	316810..316935	+	126	Protein_308	IS481 family transposase	-
CDO25_RS25210	317094..318290	-	1197	WP_088201282.1	IS256 family transposase	-
CDO25_RS25215	318382..319161	-	780	WP_010974982.1	methyltransferase domain-containing protein	-
CDO25_RS25220	319165..319965	-	801	WP_010974981.1	ABC transporter ATP-binding protein	-
CDO25_RS25225	319962..321005	-	1044	WP_010974980.1	iron ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	htpB	1..1681904	1681904

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-91)

Antitoxin

• Antitoxin ParD (3-78)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11473.15 Da
• Isoelectric Point: 10.6593

>T77517 WP_010974986.1 NZ_CP021820:c315953-315657 [Sinorhizobium meliloti]
MATERPFRLAPTAKADLRKIWRYTARRWSLEQAETYQDQLYTAFEGLAVGTKKGRNVDVRPGYLKYPAGAHIVYFRDRGD
RIDIIRILHGRVDAQRHL

Nucleotide

• Length: 297 bp

>T77517 NZ_CP021820:c315953-315657 [Sinorhizobium meliloti]
ATGGCGACTGAACGGCCTTTCCGGCTCGCTCCTACCGCCAAGGCCGACCTTCGGAAGATATGGCGCTACACGGCTCGCCG
CTGGTCTCTGGAACAGGCGGAGACCTACCAAGACCAGCTTTATACTGCATTTGAGGGACTGGCCGTAGGGACGAAGAAAG
GCCGCAATGTCGATGTGCGGCCGGGTTATCTCAAATACCCGGCAGGAGCGCACATCGTCTATTTTCGGGATCGCGGCGAC
CGGATCGACATCATCCGCATCCTGCATGGCCGTGTGGACGCCCAACGGCATTTGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8888.73 Da
• Isoelectric Point: 4.2176

>AT77517 WP_003527850.1 NZ_CP021820:c316191-315943 [Sinorhizobium meliloti]
MAARSTSISLGDHFAGFIDSQVQTGRYGSASDVVRAGLRLLEEHEAKVRALEAALIEGEESGEPEPFDNEAFKAEMRAEY
GD

Nucleotide

• Length: 249 bp

>AT77517 NZ_CP021820:c316191-315943 [Sinorhizobium meliloti]
ATGGCTGCTAGAAGCACATCTATTTCCCTTGGCGATCACTTCGCCGGTTTCATCGACAGCCAGGTGCAAACCGGCCGCTA
CGGCTCCGCCAGCGACGTTGTGCGGGCTGGACTTCGGCTTTTGGAAGAACACGAGGCGAAGGTAAGGGCGCTGGAAGCGG
CTTTGATCGAGGGCGAAGAATCCGGCGAGCCAGAGCCATTCGACAACGAAGCGTTCAAAGCCGAAATGCGCGCCGAATAT
GGCGACTGA

Structures

Toxin

• AlphaFold DB: Q92X99

Antitoxin

• AlphaFold DB: H0FXK0