77516

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 737036..737721 Replicon plasmid psymA
Accession NZ_CP021819
Organism Sinorhizobium meliloti strain M162

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag CDO25_RS22785 Protein ID WP_088201154.1
Coordinates 737302..737721 (+) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag CDO25_RS22780 Protein ID WP_088201153.1
Coordinates 737036..737305 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDO25_RS22750 732554..733423 - 870 WP_014528623.1 aldo/keto reductase family oxidoreductase -
CDO25_RS22755 733540..734463 + 924 WP_088201152.1 LysR family transcriptional regulator -
CDO25_RS22770 735443..736318 + 876 WP_164825371.1 LysR family transcriptional regulator -
CDO25_RS22775 736406..736897 - 492 Protein_700 LysR family transcriptional regulator -
CDO25_RS22780 737036..737305 + 270 WP_088201153.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CDO25_RS22785 737302..737721 + 420 WP_088201154.1 type II toxin-antitoxin system VapC family toxin Toxin
CDO25_RS22790 737875..738289 - 415 Protein_703 hypothetical protein -
CDO25_RS22795 738565..738876 + 312 WP_088198014.1 type II toxin-antitoxin system HigB family toxin -
CDO25_RS22800 738894..739274 + 381 WP_003526626.1 transcriptional regulator -
CDO25_RS22805 739534..740535 + 1002 WP_088201155.1 IS30 family transposase -
CDO25_RS22810 740661..740993 + 333 Protein_707 helix-turn-helix domain-containing protein -
CDO25_RS22815 740994..741236 - 243 Protein_708 SDR family NAD(P)-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - virB4 1..892745 892745
- flank IS/Tn - - 739534..740535 1001


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-124)

Antitoxin

SpoVT-AbrB domain

(18-55)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 14804.13 Da        Isoelectric Point: 4.8721

>T77516 WP_088201154.1 NZ_CP021819:737302-737721 [Sinorhizobium meliloti]
VSRLYMLDTNIVSELARNPQGPVTKRIAEVGPDAICVSIITAAELRYGCAKKGSSKLLAQIETILGSMQVLALDVPADAE
YGGIRAELEAAGKPIGPNDLFIAAHACMLGAVLVTANSSEFTRVRDLKVENWLDFTPSC

Download         Length: 420 bp

>T77516 NZ_CP021819:737302-737721 [Sinorhizobium meliloti]
GTGAGCAGGCTCTACATGCTGGACACCAATATCGTGTCCGAGCTTGCGCGAAACCCACAGGGGCCTGTCACTAAACGCAT
TGCCGAGGTAGGACCGGATGCAATCTGCGTCAGCATCATTACGGCGGCAGAGTTACGCTATGGATGCGCCAAGAAGGGAT
CATCGAAACTACTGGCGCAGATCGAGACCATTCTTGGAAGTATGCAAGTGCTTGCGCTCGATGTGCCTGCTGATGCCGAA
TACGGCGGCATCCGGGCTGAACTGGAGGCCGCTGGTAAGCCCATCGGCCCGAATGACCTGTTCATCGCCGCTCATGCCTG
TATGCTTGGAGCGGTGCTGGTAACAGCCAACAGTAGCGAGTTCACACGCGTTCGTGATCTCAAGGTCGAGAACTGGCTGG
ATTTCACTCCTTCGTGTTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10217.70 Da        Isoelectric Point: 4.4798

>AT77516 WP_088201153.1 NZ_CP021819:737036-737305 [Sinorhizobium meliloti]
MPELRHNEPTPPREAKLFRNNRSQAVRIPADFELPGDRVMIHRDGDRLIIEPVLRKNLLEVLAGLEPLGSEDEFPDLDET
LLPVKEIDL

Download         Length: 270 bp

>AT77516 NZ_CP021819:737036-737305 [Sinorhizobium meliloti]
ATGCCCGAACTCCGACACAACGAACCAACACCGCCAAGGGAAGCTAAGCTCTTCCGAAATAATAGAAGTCAGGCTGTACG
TATCCCCGCTGATTTTGAGCTTCCCGGCGATCGGGTGATGATCCATCGGGACGGAGATCGGCTGATAATAGAGCCGGTGC
TCCGCAAGAACTTGCTGGAGGTGCTGGCTGGTTTGGAGCCGCTCGGGTCTGAAGATGAGTTCCCTGACCTTGACGAAACC
CTGCTGCCGGTGAAGGAAATCGACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References