Detailed information of TA system TA077515

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 97296..97894
• Replicon: plasmid psymA
• Accession: NZ_CP021819
• Organism: Sinorhizobium meliloti strain M162

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CDO25_RS19465
• Protein ID: WP_088200874.1
• Coordinates: 97601..97894 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q930F1
• Locus tag: CDO25_RS19460
• Protein ID: WP_010967242.1
• Coordinates: 97296..97604 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO25_RS19420	92456..93394	+	939	WP_088200872.1	nucleoside hydrolase	-
CDO25_RS19425	93391..94917	+	1527	WP_088200873.1	amidohydrolase	-
CDO25_RS19430	95108..95431	-	324	WP_003526806.1	DNA-binding transcriptional regulator	-
CDO25_RS19435	95421..95780	-	360	WP_014989665.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CDO25_RS34110	96150..97101	+	952	Protein_96	ParB N-terminal domain-containing protein	-
CDO25_RS19460	97296..97604	+	309	WP_010967242.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
CDO25_RS19465	97601..97894	+	294	WP_088200874.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDO25_RS19470	98156..99355	-	1200	WP_198388822.1	NAD-dependent formate dehydrogenase	-
CDO25_RS34925	99726..100517	+	792	WP_088201226.1	ParB N-terminal domain-containing protein	-
CDO25_RS19480	100895..101671	-	777	WP_014528764.1	histidinol-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	virB4	1..892745	892745

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-53)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11277.99 Da
• Isoelectric Point: 8.5037

>T77515 WP_088200874.1 NZ_CP021819:97601-97894 [Sinorhizobium meliloti]
MRLVWARYALDDRDTIFSYIERENPRAAVHVDEEIVSAVRRLLDFPESGRPGRIAGTRELVIPRTPYIAAYMVMKDRIRI
LRVLHGAQKWPSELDDG

Nucleotide

• Length: 294 bp

>T77515 NZ_CP021819:97601-97894 [Sinorhizobium meliloti]
ATGAGGCTCGTCTGGGCGCGATATGCGCTTGACGATCGCGACACCATCTTCAGTTACATCGAAAGGGAGAATCCGAGGGC
GGCGGTCCACGTCGACGAGGAGATCGTCAGCGCGGTACGTCGTCTGCTTGATTTTCCGGAAAGCGGCCGCCCTGGCCGGA
TTGCCGGAACTCGAGAGCTTGTTATCCCTCGGACCCCCTACATTGCAGCCTACATGGTAATGAAGGATAGAATTCGAATT
CTGCGTGTCCTGCACGGCGCCCAGAAGTGGCCCAGCGAGCTCGACGACGGGTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11210.58 Da
• Isoelectric Point: 5.6604

>AT77515 WP_010967242.1 NZ_CP021819:97296-97604 [Sinorhizobium meliloti]
MASNALVQTRIDAEVKERATAVLENMGLTVSDAVRILLTRTANEGALPLELFSHSEAHDAWFRAKVLRALEDTRPDVDDA
DADAHFRERRAAALRKAAAGDR

Nucleotide

• Length: 309 bp

>AT77515 NZ_CP021819:97296-97604 [Sinorhizobium meliloti]
ATGGCTTCAAATGCACTTGTTCAGACTCGAATCGATGCCGAGGTCAAAGAGCGCGCCACGGCGGTTTTAGAGAACATGGG
TCTGACGGTCTCGGACGCTGTTCGAATCCTGTTAACTCGGACGGCGAATGAGGGCGCCCTGCCGCTGGAACTCTTCAGCC
ACAGCGAGGCGCACGACGCTTGGTTCCGCGCCAAAGTGTTGCGGGCACTCGAGGATACCCGACCCGACGTCGACGATGCC
GACGCCGATGCGCACTTTCGAGAGCGCCGGGCGGCAGCTTTGCGCAAGGCTGCGGCGGGTGATCGATGA

Structures

Antitoxin

• AlphaFold DB: Q930F1